ARTICLE

Enantiomer and Topoisomer Separation of Acidic Compounds on Anion-Exchanger Chiral Stationary Phases by HPLC and SFC
Pilar Franco Tong Zhang, Andrea Gargano Marek Mahut, Michael Lammerhoffer and Wolfgang Lindner
Quinine- and quinidine-derived anion-exchanger chiral stationary phases have been proved to be versatile in enantiomer separation of acidic compounds in HPLC. In this article, the authors demonstrate their performance in specific HPLC applications involving enantiomer resolution and topoisomer separation. The extension of their applications from HPLC to supercritical fluid chromatography (SFC) is also investigated with the purpose of assessing the influence of a series of parameters and gaining insight into the general approaches for SFC method development and optimisation.

An Improved Liquid Chromatography Tandem Mass Spectrometry Method to determine Clenbuterol in Human Urine
Jianghai Lu, Genye He, Xiaobing Wang, Youxuan Xu, Sheng Yang, Ying Dong, Yun Wu, Zhiyong Yang, Moutian Wu, Gangfeng Ouyang
An improved liquid chromatography tandem mass spectrometry (LC–MS–MS) method for the screening and confirmation of clenbuterol in human urine in sports doping applicatiosns was developed and validated.

COLUMNS

LC Troubleshooting
Evaluation of an LC Method
Fatos Osmani and John W. Dolan
How can we tell if an LC method is working properly? How can a compendial method be adjusted to speed up the run time?

GC Connections
Q & A on Gases for GC
John Hinshaw
In this month's installment, we address common questions about various gases and their delivery to a gas chromatograph.

Questions of Quality
Getting It Write
Bob McDowall
A look at some pitfalls to avoid when writing procedures.

The Essentials
Quadrupole Mass Analyzers and MS-MS Analysis
A very useful overview is presented of the working principles of the most popular of all mass analyzing devices and an introduction to the use of quadrupole devices in MS–MS analysis.

HPLC 2013 Preview
A look at what's on offer at next year's HPLC 2013, in Amsterdam, which is co-chaired by Peter Schoenmaker and Wim Kok.

DEPARTMENTS:
Products.
Events

Webinar: Pesticide Analysis Using Triple Quadrupole GC-MS/MS
Learn about optimization of GC and MS conditions and tools and practices to achieve rugged results in pesticide residue analysis in food. The webinar will also demonstrate the benefits of using backflushing, PTV injection, analyte protectants and internal standards in routine analysis, using examples obtained with the optimized method in USDA Pesticide Data Program labs. Register now

The Growing Relevancy of Sample Preparation in Food and Environmental Monitoring.
Thursday, October 25, 2012, 9:00 AM EDT; 15:00 CEST
Increasing global food exports, numerous food contamination scandals and trends towards stronger environmental regulation have driven the growth in food and environmental sample analysis dramatically in recent years
Read more