July 2012 Subscribe Go to Application Note Library High-Throughput, High-Efficiency Metabolome Profiling Using the Agilent 6550 iFunnel Q-TOF LC–MS System Nicola Zamboni, PhD, Institute of Molecular Systems Biology ETH Zurich, Switzerland and Agilent Technologies The Agilent 6550 iFunnel Q-TOF system has been coupled with flow injection analysis to provide profiling of small molecules in 96 biological extracts. This experiment demonstrates the superior performance of this platform, compared to the results produced with a previous generation Q-TOF instrument.   A Q-TOF Generated, Metabolomics-Specific LC–MS-MS Library Facilitates Identification of Metabolites in Malaria Infected Erythrocytes Theodore R. Sana, PhD, Agilent Technologies This study demonstrates the use of Agilent’s new metabolomics focused MS-MS spectral library, the METLIN PCDL, to elucidate the identification of several amino acids that are important in malaria metabolism.   Mass Profiler Professional and Personal Compound Database and Library Software Facilitate Compound Identification for Profiling of the Yeast Metabolome Stefan Jenkins, PhD, Agilent Technologies A nontargeted, discovery-based approach for analyzing the yeast metabolome in response to calcium and immunosuppressant drug treatment was demonstrated. Differential analysis between control and treated groups yielded many significant compounds. Database searching using the Agilent METLIN compound database and library resulted in putative identifications of many differential compounds. Simultaneous Qualitative and Quantitative Analysis of Buspirone and its Metabolites with the Agilent 6550 iFunnel Q-TOF LC–MS System Yuqin Dai, PhD, Agilent Technologies This note describes an integrated qual/quan workflow and the utility of the Agilent 6550 iFunnel Q-TOF LC–MS system for the determination of metabolic stability, metabolite identification, and metabolite profiling in an in vitro buspirone (1 μM) metabolism study in rat liver microsomes.   Quatitation of Cystine and Identification of Related Metabolites in White Blood Cells Using a High Resolution Accurate Mass LC–MS Approach Na Pi Parra, PhD, Agilent Technologies A high-resolution accurate mass LC–MS approach was demonstrated for quantitation and profiling of small molecule metabolites in complex biological samples. Excellent assay performance was achieved in the quatitation of cystine using the ultra-high resolving power and mass accuracy of an Agilent 6530 Q-TOF system.   Automated Online Card Extraction LC–MS System for the Determination of Clozapine and its Metabolites in Rat Blood Lester Taylor, PhD, Agilent Technologies This application note describes the development and validation of a method on the online analysis of dried blood spot cards for the quantitation of clozapine and metabolites. The quantitative performance of this method was evaluated and compared to that of a conventional offline extraction hole punching method. High Sensitivity SISCAPA-Based Peptide Quantitation Using UHPLC and the 6490 QQQ with iFunnel Technology Leigh Anderson, PhD, SISCAPA Assay Technologies and Agilent Technologies Quantitation of peptides by MRM MS allows specific, internally-standardized measurement of biomarkers in digests of plasma, and can achieve sub-ng/mL detection levels. In this work, LC–MS analysis was performed using a more robust standard-flow LC method to achieve comparable results to nanoflow assays.   Fast Analysis of Vitamin D from Serum or Plasma Dennis Nagtalon, Agilent Technologies The analysis of vitamin D in a clinical research environment shows a quick and simple analytical method for rapid sample turnaround. LC–MS is ideal due to its fast analysis time and high-throughput capabilities with the ability to quantify both vitamin D forms.   Screen and Quantify Pain Management Drugs for Forensic Toxicology Peter Stone, PhD, Agilent Technologies LC–MS is an ideal solution for accurately and confidently analyzing large panels of pain management compounds spanning multiple drug classes. A simple method is presented for the comprehensive LC–MS analysis of 65 pain management drugs and their metabolites in urine, with rapid sample turnaround for high throughput.   Two Minute Quantitative Analysis of Immunosuppressant Drugs Linda Cote, Agilent Technologies A quick and simple method for rapid turnaround of immunospuppressants using LC–MS in clinical research is shown, which is an ideal solution for routine analysis of target compounds such as cyclosporine A.  It delivers the sensitivity and accuracy along an alternative to time consuming sample preparation.