June 2012 Subscribe Go to Application Note Library Surfactants Quantitation Using Surfactant Plus Column on HPLC-CAD Marc Plante, Bruce Bailey, Ian Acworth, and Christopher Crafts, Thermo Fisher Scientific A multitude of surfactant types (cationic, anionic, non-ionic, zwitterionic, and polymeric) can be easily quantified using the specifically-designed Thermo Scientific Acclaim Surfactant Plus HPLC column combined with the sensitivity of charged aerosol detection.   Carbohydrates Analysis Using HPLC with PAD, FLD, CAD and MS Detectors Bruce Bailey, Paul Ullucci, Rainer Bauder, Marc Plante, Chris Crafts, and Ian Acworth, Thermo Fisher Scientific Measure carbohydrates by several different HPLC methods using various detector strategies (pulsed amperometric electrochemical, fluorescence following derivatization, charged aerosol detection, and mass spectrometry). Sensitive Analysis of Underivatized Amino Acids and Peptides Using UHPLC with Charged Aerosol Detection Christopher Crafts, Marc Plante, Bruce Bailey, and Ian Acworth, Thermo Fisher Scientific More than 18 amino acids were resolved in less than 9 min with sensitivity to low nanogram levels.  Reaction monitoring of a tryptic digest yielded mass balance recovery results within 12% of the expected value and far greater accuracy than traditional UV techniques.   Analytical Methods to Qualify and Quantify PEG and PEGylated Biopharmaceuticals Christopher Crafts, Bruce Bailey, Marc Plante, and Ian Acworth, Thermo Fisher Scientific Quantify both the PEGylated product and residual PEGylation reagents in less than 20 min using a 2D LC configuration. On-line software controlled sample preparation allowed for monitoring the degree of PEGylation. Simplifying Cleaning Validation via HPLC with Charged Aerosol Detection Christopher Crafts, Marc Plante, Bruce Bailey, and Ian Acworth, Thermo Fisher Scientific Charged aerosol detection provides a more accurate profile of residual content than traditional UV approaches.  A non-specific fast screening approach and a semi-quantitative analysis of unknowns are presented.   Easy Quantification of Organic and Inorganic Counter Ion Salts Christopher Crafts, Bruce Bailey, Marc Plante, and Ian Acworth, Thermo Fisher Scientific Improvements in column technology and detection techniques enable the simultaneous analysis of 23 compounds consisting of a mix of APIs along with anionic and cationic counter-ions. The quantification of counter-ion content for several APIs are discussed.   Fast, Sensitive (U)HPLC Methods for Tetracycline Drugs Suparerk Tukkeeree and Jeff Rohrer, Thermo Fisher Scientific The results from both methods meet or exceed the criteria in the appropriate United States Pharmacopeia (USP) monographs. Both methods are faster and show higher resolution than the current methods. These methods may be applicable to other TC-HCl-containing drug products. Determination of Beer Polyphones, Proanthocyanidins and Bitter Acids Using Spectro-Electro Array Technology Paul Ullucci, Ian Acworth, Marc Plante, Bruce Bailey, and Christopher Crafts, Thermo Fisher Scientific Determine 40 different polyphenols, alpha and beta bitter acids, and cis-and trans-isobitter acids in beer using this gradient HPLC-DAD-ECD method.  Principal component analysis (PCA) could readily distinguish between different beer types. Quantitation of Hindered Amine Light Stabilizers (HALS) Marc Plante, Bruce Bailey, and Ian Acworth, Thermo Fisher Scientific Complex polymer additives, hindered amine light stabilizers (HALS), may not ionize (MS) or absorb UV.  An HPLC method using charged aerosol detection is described providing chromatographic conditions for these challenging analytes. Simple HPLC Method for Total Glycerides in All Biodiesels Marc Plante, Bruce Bailey, Ian Acworth, and Christopher Crafts, Thermo Fisher Scientific A simple but sensitive HPLC method quantifies total glycerides in any biodiesel sample, including in-process, washed, and finished B100, as well as petroleum-biodiesel fuel mixes (B1– B99). Analyze all samples even when GC methods cannot.