July 2012
Subscribe Go to Application Note Library

Food-and-Beverage
Determination of Mono-, Di-, and Triphosphates and Citrate in Shrimp by Ion Chromatography
Chanita Chantarasukon, Suparerk Tukkeeree, and Jeffrey Rohrer, Thermo Fisher Scientific
Polyphosphates are legally added to some food products such as meat and seafood to improve the appearance and texture of the product and increase the weight of the seafood. Ion chromatography (IC) is commonly used to determine large and small polyphosphates, as well as citrate.
 
Determination of Trace Sodium in Cranberry Powder
Brian DeBorba and Jeffrey Rohrer, Thermo Fisher Scientific
Cranberry is one of the five fastest growing super fruits with sales of cranberry supplements increasing by about 30% in 2010 since their reported ability to combat urinary tract infection, particularly in women. Cranberry powder contains organic acids, proanthocyanidins, and polyphenols, this application describes the determination of organic acids in cranberry extracts.
 
HPLC Analysis of Six Active Components of Caulis Lonicerae Using a Phenyl-1 Column
Xu Qun, Huang Xiongfeng, and Jeffrey Rohrer, Thermo Fisher Scientific
Caulis Lonicerae is an important traditional Chinese medicine used for the treatment of acute fever, headache, respiratory infection, and epidermic diseases.  This efficient and comprehensive HPLC QC method separates the six main active components of Caulis Lonicerae: loganin, sweroside, chlorogenic acid, caffeic acid, rutin, and galuteolin.
 
Determination of Phytic Acid in Soybeans and Black Sesame Seeds
Thunyarat Phesatcha, Suparerk Tukkeeree, and Jeffrey Rohrer, Thermo Fisher Scientific
Phytic acid is stored in the plant tissues of seeds and grains and its content levels in foods is important because it is not digested by humans and can prevent the absorption of iron and calcium by the intestine, even though as an antioxidant it can prevent carcinogenesis.
 
HPLC Separation of All-Trans-ß-Carotene and its Iodine-Induced Isomers Using a C30 Column
Chen Jing, Xu Qun, and Jeffrey Rohrer, Thermo Fisher Scientific
Carotenoids, ß-Carotene being the most commercially important, have protective effects against serious disorders such as cancer, heart disease, and degenerative eye disease.  In this method, a longer C30 alkyl chain of the stationary phase was ideal for separating hydrophobic structurally related isomers.
 
Mogroside V Determination by HPLC with Charged Aerosol and UV Detections
Deanna Hurum and Jeff Rohrer, Thermo Fisher Scientific
Luo han kuo fruit has long been used in traditional Asian medicine because of numerous potential health benefits such as antioxidant activity, anticancer effects, and anti-hyperglycemic effects. The glycosides have also been isolated because they are intensely sweet and have been investigated as sugar substitutes and flavor enhancers.
 
A Validated Method of Measuring Lactose in Milk
Thermo Fisher Scientific
Lactose, the major carbohydrate in milk, contributes to the sensory and functional properties of milk and has economic value since the price of milk is based on milk solids content. Measurement of milk's physical properties is used to determine the concentration of milk components and the quality of milk products.
 
Determination of Iodide in a Nutritional Supplement Using an Acclaim Mixed-Mode WAX-1 Column
Thermo Fisher Scientific
The use of iodinated salt is the most frequent way of obtaining the essential micronutrient iodide. Ion chromatography (IC) is a commonly used for determining iodide but some samples need an HPLC method.  This application discusses two reversed phase C18 methods to determine iodide.