Application Note Alert
February 2012 Subscribe

Environmental
Fast Separation of Seven Biocides Using an Agilent ZORBAX Rapid Resolution High Definition Eclipse Plus C18 1.8 µm Column
Judy Berry and John W. Henderson Jr., Agilent Technologies
An Agilent ZORBAX rapid resolution high definition (RRHD) Eclipse Plus C18 1.8 μm column separated seven different biocides rapidly with high resolution. The Agilent 1290 Infinity LC system was used. Methylparaben, listed as an Echinacea supplement ingredient, was easily identified and a hand sanitizer containing 2-phenoxyethanol and methylparaben also were easily and rapidly identified using the method.

Food-Beverage
Analysis of Pesticide Residues in Apples Using Agilent SampliQ QuEChERS AOAC Kit by LC–MS-MS Detection
Limian Zhao, David Schultz, and Joan Stevens, Agilent Technologies
A method using Agilent’s QuEChERS for sample preparation, and LC-ESI MS-MS, together with the ZORBAX Phenyl-Hexyl column is used to analyze 16 pesticides in apples.
 
Analysis of Pesticide Residues in Green Tea Using Agilent QuEChERS and LC–MS-MS Detection
Chen-Hao Zhai, Agilent Technologies Co., Ltd.
An analysis of 12 pesticides in green tea, taking a time-consuming SPE sample clean-up method and revising it to use QuEChERS for substantial time savings, while maintaining good limits of quantitation. A Poroshell 120 column was used for the analysis.
 
Determination of Benzimidazole Fungicides in Apple Juice by Agilent Polymer SCX Solid-Phase Extraction with High-Performance Liquid Chromatography
Chen-Hao Zhai and Yun Zou, Agilent Technologies Co., Ltd.
Solid-phase extraction (SPE) coupled with high-performance liquid chromatography (HPLC) was optimized for extraction and quantification of two fungicides (carbendazim and thiabendazole) in apple juice. Results indicate that SPE using Agilent SCX (60 mg, 3 mL) and HPLC using an Agilent ZORBAX Eclipse Plus C18 column (4.6 mm × 100 mm, 3.5 μm) is an excellent combination for extraction and analysis of these compounds. Recoveries ranged from 92.1% to 99.4% with RSDs below 5% and limits of detection of 4 μg/kg.
 
Multiresidue Analysis of 100 pesticides in Food Samples by LC/Triple Quadrupole Mass Spectrometry
Imma Ferrer and E. Michael Thurman, Pesticide Residue Research Group, University of Almeria; Yanyan Fang, Paul Zavitsanos, and Jerry A. Zweigenbaum, Agilent Technologies, Inc.
A method for confirming a group of 100 pesticides in vegetable and fruit samples was developed using the Agilent G6410AA Triple Quadrupole MS. The sensitivity obtained meets the maximum residue levels (MRLs) established by the European Union regulation. The analytical performance of the method was evaluated for different types of fruit and vegetables — orange, tomato, and green pepper — showing little or no matrix effects. A ZORBAX Eclipse XDB C8 column was used, and Agilent QuEChERS was used for sample preparation.
 
Fully Automated QuEChERS Cleanup and LC–MS-QQQ Analysis of Pesticides in Fruits and Vegetables
Paul H. Robert, Anatune Ltd.
An automated dispersive solid phase extraction (DSPE) is conducted for cleanup, using a combination of primary secondary amine (PSA) to remove fatty acids and anhydrous magnesium sulphate to reduce the remaining water in the extract. After mixing and centrifugation the upper layer is ready for analysis.
 
Multiresidue Analysis of 301 Pesticides in Food Samples by LC/Triple Quadrupole Mass Spectrometry
E. Michael Thurman and Imma Ferrer, Department of Civil, Environmental and Architectural Engineering Center for Environmental Mass Spectrometry, University of Colorado; and Jerry A. Zweigenbaum, Agilent Technologies, Inc.
An analytical methodology for screening and confirming the presence of 301 pesticides in vegetable samples was developed using the Agilent G6410A Triple Quadrupole mass spectrometer (QQQ). We found that 90% could be identified using this procedure with a limit of detection (LOD) in vegetable matrices of 0.01 mg/kg (ppm) or below. A ZORBAX Eclipse Plus RRHT SB-C18 column was used.
 
Analysis of Color Additives in Sweets
Siji Joseph, Agilent Technologies, Inc.
A robust HPLC method for simultaneous determination of 10 synthetic colorants is discussed in detail. Separation and quantification were achieved with an Agilent 1260 Infinity LC System using an Agilent Poroshell EC-C18 column. The HPLC method was effectively transferred to a short UHPLC method using an Agilent 1290 Infinity LC System for faster analysis without compromising resolution.

General
Analysis of Bisphenol A Leaching from Baby Feeding Bottles
Syed Salman Lateef, Agilent Technologies, Inc.
Bisphenol A can leach out from the plastic surfaces of food containers and has been detected in various matrices such as plasma, urine, and groundwater. We describe a method to quantify bisphenol A and a structurally similar analog bisphenol F extracted from baby feeding bottles. The method was developed on an Agilent 1260 Infinity LC system using an Agilent ZORBAX Eclipse Plus C18 column.

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