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Analysis of Acharicides in Honey
Angelika Gratzfeld-Huesgen, Agilent Technologies
Varroosis is a bee disease caused by the mite Varroa Jacobsoni which endangers beekeeping all over the world. In order to prevent economic losses beekeepers treat their colonies with acaricides. The use of these acaricides inside beehives implies a risk of direct pollution of honey and other hive products; therefore maximum residue levels on honey have been fixed in many countries in order to protect consumers. In this application note, a HPLC method was developed and validated for four acaricides using the Agilent 1260 Infinity LC system. Further, an UHPLC method was developed using the Agilent 1290 Infinity LC system, which saves time and solvent consumption and results in better sensitivity. |
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Analysis of Natural and Artificial Vanilla Preparations
Angelika Gratzfeld-Huesgen, Agilent Technologies
Typical artificial vanilla flavorings contain synthetically produced vanillin, ethyl vanillin, vanillin mandelic acid, and piperonal. The vanillin compound used in these artificial flavorings is usually synthesized from cheap raw material, such as guaiacol, eugenol, or lignin. These products can be found in the final flavoring preparation at trace levels. In this application solution, a conventional HPLC method was developed and validated for several natural and artificial vanilla flavorings using the Agilent 1260 Infinity LC system. In addition, an UHPLC method was developed using the Agilent 1290 Infinity LC system, saving time and reducing solvent consumption. |
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Analysis of Ascorbic Acid, Citric Acid and Benzoic Acid in Orange Juice
Syed Salman Lateef, Agilent Technologies
Antioxidants, such as ascorbic acid, prevent oxidation by decreasing the available oxygen in the environment. Preservatives, such as citric acid or benzoic acid, prevent or inhibit the growth of microorganisms in food. In this application solution, we describe a method to quantify an antioxidant (vitamin C) and preservatives (citric acid, benzoic acid) in orange juice. The method was developed on the Agilent 1260 Infinity LC System using an Agilent Poroshell EC-C18 column. The method was effectively converted to a short UHPLC method using an Agilent 1290 Infinity LC system. |
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Analysis of Fat-Soluble Vitamins from Food Matrix for Nutrition Labeling
Siji Joseph, Agilent Technologies
Vitamins are nutrients required in trace amounts by an organism for its healthy growth and must be obtained from the diet. As the level of vitamins present in a food may vary from nanograms to milligrams, labeling these vitamins is a mandatory requirement by the FDA. In this application solution, a robust reversed-phase high performance liquid chromatographic method for simultaneous determination of nine fat soluble vitamins was developed. Finally, using an Agilent 1290 Infinity UHPLC system, this HPLC method was effectively transferred to a short ultra high pressure liquid chromatographic method. |
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Analysis of Water Soluble Vitamins from Multi Vitamin Tablets for Nutrition Labeling
Siji Joseph, Agilent Technologies
An application to carry out qualitative and quantitative analysis of water-soluble vitamins is described. Separation and quantification was done on an Agilent 1260 Infinity LC system using a Poroshell EC-C18 column and UV detection. Finally, this method was effectively transferred to a fast UHPLC method using an Agilent 1290 Infinity LC system. |
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Determining Total Sugar Content in Maple Syrup to Meet FDA Nutrition Labeling Requirements
Syed Salman Lateef, Agilent Technologies
Nutrition labels on food items provide valuable information to consumers. Therefore, to ensure authenticity, the Food and Drug Administration (FDA) checks the reported values on these nutrition labels. This Agilent application solution demonstrates a sucrose analysis method for commercially available maple syrup using the Agilent 1260 Infinity Binary LC system coupled to the Agilent 1260 Infinity Refractive Index Detector. |
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Mackerel Pesticides Analyzed by Gel Permeation Chromatography with Agilent EnviroPrep
Greg Saunders and Ben MacCreath, Agilent Technologies
As part of a food safety program, it was necessary to assess the level of the pesticide 1,2,3,4,5,6-C6H6Cl6 in a sample of seafood. Gel permeation chromatography (GPC) was used because GPC is a simple technique for cleaning up food and environmental samples. Agilent EnviroPrep columns were selected for the separation. |
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Alkyl Glycerides from Frying Fat on Agilent PLgel 3 µm with Gel Permeation Chromatography
Greg Saunders and Ben MacCreath, Agilent Technologies
Alkyl glycerides are discrete molecules commonly found in frying fats and oils. Agilent's PLgel 3 µm 100Å columns for gel permeation chromatography have been specifically designed for the analysis of low molecular weight molecules from complex mixtures such as these. In this example, the columns are used for the analysis of alkyl glycerides, and for resolving individual molecules from complex alkyl glyceride mixtures such as occur in frying fats. |
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Identification of Different Meat Species by the Agilent Fish ID Solution on the Agilent 2100 Bioanalyzer
Steffen Mueller, Agilent Technologies
In food processing plants, a large number of various ingredients originating from different animal species are used as part of the production process. Sometimes this is done on the same production line. The Agilent Fish ID solution was developed to identify the species of fishes from food samples of different processing levels. This application note investigates its abilities to detect the presence of non-fish species, specifically mammalian or avian DNA originating from dairy products or meat. |
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