Fast and Automatic Mapping of Disulfide Bonds in a Monoclonal Antibody using SYNAPT G2 HDMS and BiopharmaLynx 1.3 Hongwei Xie and Weibin Chen, Waters Corporation
In this study, an advanced LC–MSe peptide mapping workflow is demonstrated for the detection and identification of disulfide linkages, including scrambled disulfide linkages, in a recombinant IgG1 mAb. We develop a viable, integrated approach for automatic mapping or monitoring of disulfide bonded peptides in a protein with multiple cysteines.
