GPC HPLC GC Sample Prep Hyphenated General May 2012 Subscribe Go to Application Note Library Featured Application Note: Preservative Compounds Found in Cosmetics Joanna Freeke, Thermo Fisher Scientific Preservatives are added to cosmetics to ensure they don’t carry pathogenic microorganisms and to eliminate growth of microbes. Cosmetic manufacturers need to screen for preservatives, analysis is becoming increasingly important because of a rising public awareness of additives affecting health. Molar Mass Determination of Gelatins Daniela Held and Günter Reinhold, PSS-Polymer Standards Service-USA Inc. Reproducible and easy-to-use GPC/SEC analysis for all types of gelatins can be done using a SECcurity GPC system, PROTEEMA columns, and a solvent with a pH of 5.5 for acidic and 6.6 for basic produced gelatin.   Determination of Pesticides in Red Wine by QuEChERS Extraction, Quick QuEChERS Clean-up, and LC–MS-MS Detection Xiaoyan Wang and Don Shelly, UCT, Inc. Red wine contains pigments such as anthocyanins, and a mixture of organic acids, sugars, and phenols. This complex matrix presents an analytical challenge for the determination of trace pesticide residues. Use of the UCT Quick QuEChERS push thru cartridge ECPURMPSMC after QuEChERS extraction provides a simple, fast, novel, and effective method for clean-up. Screening for Chemical Residues in Food Using Accurate Mass Technology and Intelligent Software Solutions Andre Schreiber and Carmai Seto, AB SCIEX We continue to be on a mission to ensure safety in our food supply, and technology is advancing to enable the screening of food for both targeted and non-targeted chemical contaminants, all in a single analysis. Here, we describe the methodology to do this analysis, with a simple sample preparation using high resolution/high accuracy mass spec analysis, and we describe unique software that allows food scientists to deeply investigate the data to get the most information from every analysis.   Comparing HILIC and RPLC of Morphine Using Agilent ZORBAX RRHD Columns with UHPLC–MS Anne. E. Mack, Agilent Technologies Hypdrophilic interaction chromatography (HILIC) is gaining popularity in liquid chromatography, particularly for its ability to retain and separate small polar analytes.  This app note evaluates three ZORBAX rapid resolution high definition (RRHD) phases to compare mass spectrometery sensitivity with reversed-phase and HILIC for this analysis.   Analysis of Phosphate Compounds with the Agilent 1260 Infinity Bio-inert Quaternary LC System Sonja Schneider, Agilent Technologies With the Agilent 1260 Infinity Bio-inert Quaternary LC system, phosphate compounds can be analyzed without any issues regarding the formation of phosphate-iron complexes found with stainless steel systems.   Determination of 4-Methylimidazole in Cola Soft Drinks Using a Purospher®STAR RP-18e Column and UV Detection Anita Piper and Patrik Appelblad, EMD Herein, a simple yet rugged analytical procedure is presented for direct analysis of 4-MI in well known cola soft drinks, and light versions of said beverages, and where no sample is preparation needed, using a high pH stable Purospher STAR C18-endcapped reversed phase (RP) column and UV detection.   HPLC Analysis of Mono- and Di-valent Metal Cations Kendra L. Cox, PhD, Hitachi High Technologies America, Inc. Many metal ions play crucial roles in biological systems. As such, it is important to accurately measure the concentration of these metal ions in various food sources. Presented here is a method for analysis of six metal ions (Li+, Na+, K+, Mg2+, Ca2+, and Zn2+) in food using the highly flexible Hitachi Chromaster HPLC system with evaporative light scattering detection.   HALO Penta-HILIC Phase Provides Improved Retention and Alternate Selectivity vs. Silica HILIC for Ten Cephalosporins MAC-MOD Analytical and Advanced Materials Technology In this example application, the performance of the HALO Penta-HILIC column is compared against the HALO HILIC (silica) column for a gradient separation of 10 cephalosporins in less than 10 min. Improved retention, peak shape, and different selectivity are observed for the Penta-HILIC phase.   Lisinopril; Fast, Reproducible, Easy & Reliable Method J.E. Young, MicroSolv Technology Corporation As a highly hydrophilic compound, Lisinopril is not well-suited to reverse phase methods. This method does not suffer from the same method drawbacks as the USP method and offers fast (8 min run, 2 min post time), repeatable (5 run overlay shown) runs with an easy to make mobile phase.   HPLC Analysis of Pre-Column Derivatized Amino Acids Shimadzu Various derivatization methods have been devised to improve sensitivity and selectivity when conducting amino acid analysis by HPLC. This application presents an analysis using pre-column derivatization with OPA and FMOC, utilizing automated pretreatment autosampler functions and fluorescence detection.   Separation of 5-fluorouracil and Its Derivatives Using a Stable Bonded Phase HILIC Column Atis Chakrabarti, Tosoh Bioscience LLC Chemotherapy agent 5-fluorouracil (5-FU) is a pyrimidine analog that has been used as an anticancer drug for more than 40 years. This application note discusses the effectiveness of a TSKgel NH2-100 HILIC column in retaining both 5-FU and 5-fluoro-2'-deoxyuridine and allowing for their separation from several derivatives using a conventional HPLC system. Sub ug/L Level Analysis of Chlorinated Pesticide and Herbicide Analysis in Water by GC/u ECD Using Agilent J&W DB35ms Ultra Inert GC Column Ken Lynam and Doris Smith, Agilent Technologies This app note demonstrates the consistent and sensitive analysis for the chlorinated compounds at and below established maximum contaminant concentrations when using a dual column GC/uECD approach using the Agilent J&W DB035msUI GC column.   Determining Phenolic Compounds in Whisky Eike Kleine-Benne, PhD and Yunyun Nie, GERSTEL LVI-GC–MS and SBSE-TD-GC–MS are evaluated for quantitative determination of seven phenolic compounds in whisky with no or little sample preparation. Whereas both techniques provide good linearity and reproducibility, SBSE delivers lower LODs and LOQs.   Advice on Sorbent Selection, Tube Conditioning, Tube Storage, and Air Sampling Elizabeth Woolfenden, Markes International Ltd. This application note gives advice on which sorbent to use for pumped or diffusive tube monitoring of various vapor-phase organics, and suggests parameters for conditioning and storing packed tubes. It goes on to describe the various types of sorbents for VOC and SVOC monitoring applications, their various properties, example analytes which each can trap, and recommended desorption temperatures.   Volatile Compounds in Beer Lee Marotta and Andrew Tipler, PerkinElmer, Inc. This application note describes a system comprising a headspace trap sampler to extract and concentrate volatile organic compounds from a beer sample and deliver them to a gas chromatograph–mass spectrometer (GC–MS) for separation, identification, and quantification. Automated Solid Phase Extraction of Semi-Volatile Organic Compounds (AB 8270 SIM) in Water Phil Bassignani, FMS, Inc. EPA Method 8270 is used to determine the concentration of semi-volatile organic compounds in extracts prepared from solid waste matrices such as soils, air sampling media, and water samples. The following application outlines the complete automation of a modified 8270 extraction, delivering a complete, out-of-the box MDL study demonstrating both precision and accuracy for mixed classes of low-level analytes. Evaluation of Sample Pretreatment Procedures for the Determination of Phthalate Esters in Child Care Products and Children's Toys by GC–MS Shimadzu The Consumer Product Safety Improvement Act of 2008 requires testing of child care products and toys in the US for specific phthalate esters by GC–MS. In this study, analysis by pyrolysis GC–MS was investigated as a technique to simplify sample preparation and improve analytical precision.   Enhanced Determination of Alcohol Biomarkets Karin Wendt, Dr. Carsten Baessmann, Thomas Zey, James Hillis PhD, and Dr. Wolfgang Weinmann, Bruker Daltonics Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are  significant clinical and forensic biomarkers because they offer an extended historical evidence trail of alcohol exposure and abuse. EtG and EtS present practical challenges in the sensitivity required for their quantitation and significant matrix effects are often observed. The application of a new ion source, in conjunction with high-performance time-of-flight mass spectrometry, provides the solution to these challenges. Identification of Nanoparticles in Instant Cappuccino Petra Krystek and Thomas Jocks, Wyatt Technology Nanoparticles have made their way into numerous consumer products that are used by hundreds of millions of people. This torrid pace of development is paralleled by the need to collect reliable data on the distribution and behavior of these particles. The characterization of nanoparticles represents a pivotal step in the quality control of the production process.