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September 2013 Pharma Biopharma Natural Products Food Industrial Quantitation of Pluronics by High Performance Liquid Chromatography and Corona Charged Aerosol Detection Marc Plante, Bruce Bailey, and Ian N. Acworth, Thermo Fisher Scientific Pluronics, like other surfactants, are challenging to analyze as each consists of a complex mixture of structurally similar analytes. They cannot be measured by UV because of the lack of a chromophore. Now, pluronics can be quantified as a single analytical peak in both standards and OTC products using charged aerosol detection. Read More Generic Method Approach for Pharmaceutical Drug Discovery and Development Using Reversed-Phase Hydrophilic Interaction Liquid Chromatography with Universal Charged Aerosol Detection Frank Steiner, Michael Heidorn, and Markus M. Martin, Thermo Fisher Scientific This generic method addresses the need to make changes during the drug development life cycle and is applicable to each new drug formulation. Using gradient reversed-phase LC in the first stage and HILIC in the second stage, both hydrophilic and hydrophobic compounds are separated in a single, short run. Read More Advantages of High-Resolution Separation Media for Monoclonal Antibody Analysis Srinivasa Rao, Yuanxue Hou, Hongmin Zhang, Ilze Birznieks, Gurmil Gendeh, Yury Agroskin, and Chris Pohl, Thermo Fisher Scientific QC and stability assessment of MAbs are very important. This work describes the benefits of applying high-resolution IEX columns with 3 μm and 5 μm particles and a bioUHPLC system to get high-efficiency MAb and protein separations. Read More Intact Protein LC–MS: How to Overcome the Challenges? Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, and Mauro DePra, Christian Ravnsborg, Remco Swart and Dafydd Milton, Thermo Fisher Scientific LC–MS analysis of proteins can be challenging. This work describes the performance of three Thermo Scientific™ EASY-Spray™ columns dedicated to intact protein LC–MS. Recovery, separation efficiency, and resolution will be demonstrated for various conditions. Read More Profiling Hoodia Extracts by HPLC with Charged Aerosol Detection, Electrochemical Array Detection, and Principal Component Analysis Ian Acworth, Bruce Bailey, Marc Plante, Qi Zhang, and David Thomas, Thermo Fisher Scientific Profile Hoodia-related products: Extracts are analyzed by HPLC-charged aerosol detection for the measurement of actives, degradation products, and impurities that may not contain a chromophore. Generate metabolite patterns that can be used to evaluate product authenticity and adulteration using HPLC-coulometric electrochemical array detection. Read More Determination of Olive Oil Adulteration by Principal Component Analysis with HPLC–Charged Aerosol Detector Data Marc Plante, Bruce Bailey, and Ian N. Acworth, Thermo Fisher Scientific A simple “dilute and shoot” HPLC-charged aerosol detection method combined with principal component analysis easily determines authentic from adulterated extra virgin olive oil (EVOO) samples. An approach to measure EVOO quality is also provided. Read More Quantitation and Characterization of Copper Plating Bath Additives by Liquid Chromatography with Charged Aerosol Detection Marc Plante, Bruce Bailey, and Ian N. Acworth, Thermo Fisher Scientific Copper plating bath additives are fully characterized using two flexible, simultaneous HPLC methods. The accelerator and suppressor are measured using charged aerosol detection; while the accelerator and leveller are measured using electrochemical detection. Read More Determination of Polyacrylic Acid in Boiler Water Using Size-Exclusion Chromatography with Charged Aerosol Detection Mark Tracy, Xiaodong Liu, Ian Acworth, and Bruce Bailey, Thermo Fisher Scientific Scale formation limits boiler efficiency and can cause major damage. It can be prevented through anti-scalants such as polyacrylic acid and needs to be measured for both process control and wastewater discharge. A sensitive and direct method can quantify low levels of PAA and resolve it from other low-molecular weight components through SEC-charged aerosol detection. Read More   Go to Application Note Library Subscribe to the Application Note Alert LCE Print Edition LCE Digital Edition