Featured Application Note:
Automated Glycan Structural Isomer Differentiation Using SimGlycan Software
Julian Saba1, Arun Apte2, Ningombam Sanjib Meitei2, and Rosa Viner1
1Thermo Fisher Scientific, San Jose, CA; 2PREMIER Biosoft, Palo Alto, CA
Here we present an automated workflow for complete structural elucidation of glycans by combining MSn fragmentation and data processing. The combination of permethylation, MSn, and SimGlycan software enabled successful identification and differentiation of various structural isomers of chicken ovalbumin released glycans. The simplified data analysis time was reduced to a matter of minutes, thus enabling truly automated, high-throughput data analysis. |
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Fast determination of residual glycerol and glycerides in biodiesel by SFC/MS using the Agilent 1260 Infinity Analytical SFC System
Maria Rambla Alegre, Melissa N. Dunkle, Gerd Vanhoenacker, Frank David, and Pat Sandra, Research Institute for Chromatography Kennedypark 26, B-8500 Kortrijk, Belgium, Martin Vollmer, Agilent Technologies, Waldbronn
Supercritical fluid chromatography (SFC with mass spectrometric detection is a valuable technique for the determination of glycerol and mono-, di-, and triglyceride impurities at trace levels in biodiesel. |
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Analysis of Microcystins in Drinking Water
KH Cha, Kefei Wang, Bruker Daltonics
This work describes an LC-MS/MS method for detection and quantification of three common cyanobacterial toxins (MC-LR, -YR, and -RR) in drinking water with direct injection. |
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Qualitative and Quantitative Analysis of β-carotene Using UPC2
Jacquelyn Runco, Lakshmi Subbarao, and Rui Chen, Waters Corporation
A UPC2 method was successfully developed to separate the three most common carotenoids in less than two minutes. This method is four times faster that traditional methods of analysis, thereby reducing organic solvent consumption by 85%. The high-throughput UPC2 method is ideally suited for laboratories routinely performing quality control and regulatory compliance monitoring where a large number of assays are required. |
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Analysis of polycyclic aromatic hydrocarbons from vehicle exhaust using TD–GC/TOF MS
Steve Smith, ALMSCO International
This Application Note describes how the combination of thermal desorption (TD) with gas chromatography (GC) and time-of-flight mass spectrometry (TOF MS), followed by data analysis using TargetView™ compound identification software, can be used to accurately identify trace-level polycyclic aromatic hydrocarbons (PAHs) in a complex vehicle exhaust particulate sample. The sensitivity and ‘classical’ spectra produced by BenchTOF-dx allow accurate identification of PAHs with near-identical fragmentation patterns, while TargetView enables GC profiles of trace-level analytes to be visualised even when hidden under much more abundant components. |
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Antibody Drug conjugate Analysis
Wyatt Technology
A novel method for Drug Antibody Ratio determinations based on SEC-MALS in conjunction with UV absorption and differential refractive index detection. |
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Automated Solid Phase Extraction of Semi-Volatile Organic Compounds (AB 8270 SIM) in Water
Phil Bassignani, FMS, Inc.
EPA Method 8270 is used to determine the concentration of semi-volatile organic compounds in extracts prepared from solid waste matrices such as soils, air sampling media and water samples. Extractions from water samples can be particularly difficult and time consuming due to the problematic analytes that need to be extracted, concentrated and analyzed as well as the large quantities of solvent the manual sample prep process requires. Laboratories are required to report analytes at lower reporting limits than previously required, often needing to employ SIM scan variations of the method. The following application outlines the complete automation of a modified 8270 extraction, delivering a complete, out-of-the box MDL study demonstrating both precision and accuracy for mixed classes of low-level analytes.
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Extraction of Cortisol From Human Saliva Using ISOLUTE®SLE+ Plates Prior to LC-MS/MS Analysis
Kerry Stephens, Gavin Jones, Biotage AB, Sweden
Cortisol is a steroid hormone that when measured from saliva can be used as an indication of stress. This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of salivary cortisol levels. Analyte recoveries achieved using this method ranged from 96-99% with RSDs below 3% (n=7) for all analytes. |
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Determination of Phthalates Leached from Toys into Artificial Saliva
Xiaoyan Wang, UCT, Inc.
Phthalates are plasticizers added to polymers to make them softer and more flexible. They are commonly used in the manufacturing of toys. Phthalates are not permanently bonded in polymers and they may be released through touching, licking, or chewing. This new method artificially reproduces the licking and chewing actions of children by shaking cut toys in artificial saliva. Following extraction, phthalate levels are determined by GC/MS. |
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EPA 8330A Analysis of Explosives Using Agilent Poroshell 120 EC-CN and EC-C18 Columns
Anne Mack, Agilent Technologies, Inc.
A group of 14 nitroaromatics and nitramines was analyzed according to EPA Method 8330A for the trace analysis of explosive residues by HPLC/UV. The analysis was improved by using superficially porous Agilent Poroshell 120 columns as compared to the traditional 5 μm columns suggested by the United States Environmental Protection Agency (EPA). Two complimentary phases were used; Poroshell 120 EC-CN and Poroshell 120 EC-C18. As recommended by the EPA, the Poroshell 120 EC-CN column was a good confirmation column when paired with a Poroshell 120 EC-C18 column due to its orthogonal selectivity. |
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Detecting Halides & Sulfur in LPGs with MultiTek
Will Donaldson, PAC
Butane is a common feed used in production and can be blended with other compounds to achieve a desired product. When the fluorides, chlorides, and sulfur in the butane react, they can produce extremely corrosive acids, which can cause gas leaks. The Antek MultiTek, when coupled with Ion Chromatography, provides sensitive and reliable analysis of halides and sulfur in liquefied petroleum gas, which allows refineries and other processes to improve plant safety and efficiency. |
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USP Analysis of Warfarin Sodium Tablets Using Agilent Poroshell 120 EC-CN and SB-C8 Columns
Anne Mack, Agilent Technologies, Inc.
Warfarin, warfarin-related compound A, and propylparaben were analyzed according to the United States Pharmacopeia (USP) analysis for warfarin sodium tablets. The chromatographic purity and assay analyses were improved by using superficially porous Agilent Poroshell 120 columns as compared to the USP-suggested 5 μm columns. Each method was adjusted within the guidelines in USP Chapter 621 toallow for time and solvent savings with the Poroshell 120 columns. All chromatographic system requirements were met with the improved superficially porous column analyses. |
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Automated Glycan Structural Isomer Differentiation Using SimGlycan Software
Julian Saba1, Arun Apte2, Ningombam Sanjib Meitei2, and Rosa Viner1
1Thermo Fisher Scientific, San Jose, CA; 2PREMIER Biosoft, Palo Alto, CA
Here we present an automated workflow for complete structural elucidation of glycans by combining MSn fragmentation and data processing. The combination of permethylation, MSn, and SimGlycan software enabled successful identification and differentiation of various structural isomers of chicken ovalbumin released glycans. The simplified data analysis time was reduced to a matter of minutes, thus enabling truly automated, high-throughput data analysis. |
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To advertise or have an application note inserted, contact:
Mike Tessalone
+1 732 346 3016 | Email
Lindsay Jones
+44 1244 629316 | Email
Valeria Curzio
+44 207 254 0685 | Email
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