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Featured Application Note:

GC-MS/MS Analysis of OCPs, PAHs, and PCBs in Environmental Samples
Inge de Dobbeleer, Joachim Gummersbach, Hans-Joachim Huebschmann, Anton Mayer, Paul Silcock, Thermo Fisher Scientific
A high performance, highly productive analysis of OCPs, PAHs, and PCBs in environmental samples is conducted through a consolidated GC-MS/MS method.  Smart software tools are integrated into the method development and analysis workflow to minimize the time needed to implement and maintain the methodology in routine. Read More
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Seamless Instrument-to-Instrument Method Transfer of the EPA Method 8330A/B for Nitroaromatics From an Agilent 1200 Series LC to the Agilent 1290 Infinity Binary LC Using ISET
Angelika Gratzfeld-Huesgen, Agilent Technologies
This application note presents an application solution for the analysis of nitroaromatic compounds. The seamless transfer of the EPA method (8330A/B) from the Agilent 1200 Series Rapid Resolution LC (RRLC) to the Agilent 1290 Infinity Binary LC using Intelligent System Emulation Technology (ISET) is shown. Read More		 Fast Separation of Pharmaceutical Anions and Cations
Terri Christison, John E. Madden, Fei Pang, and Khalil Divan, Thermo Fisher Scientific
This application note demonstrates the advantages of using walk-up capillary ion chromatography for ultrafast separations of pharmaceutical relevant anions and cations. Identify and quantify inorganic impurities essential in drug development while using the benefits of an Always On, Always Ready IC system. Read More
Fast Analysis of Fruit Juice Acids with Poroshell 120 SB-Aq
William Long, Agilent Technologies, Inc.
The acid profile can be used to identify a juice or verify its purity.  A reversed-phase HPLC method for separation of organic acids (tartaric, quinic, malic, citric, and fumaric acids) in fruit juices is demonstrated using an Agilent Poroshell 120 SB-Aq column. The chromatographic separation was performed using  potassium dihydrogen orthophosphate buffer (pH 2.5) as mobile phase and diode array detection. Read More		 SEC-MALS Characterization of Unfractionated Heparin
Lin Rao and John Beirne, Wyatt Technology
Heparin has been used as an anticoagulant for over sixty years and one of the fundamental parameters for char­acterizing unfractionated heparin (UFH) is its molecu­lar weight (MW) and MW distribution. The molecular weight of heparin ranges from 3 to 50 kDa, but, typically falls within the 10 to 20 kDa range for unfractionated heparins used in medical applications. In light of hepa­rin contamination issues in 2008, USP has proposed to include heparin molecular weight determination for the stage 3 heparin monograph revisions. Read More
 
GPC
Automated Solid Phase Extraction and Concentration of PAHs in Water
Phillip Bassignani, FMS, Inc.
Analytically, PAHs present challenges for testing labs. Their volatile nature can make them problematic to extract without loss using manual methods. This is especially true for the evaporation or concentration of sample extracts. The following procedure utilizes the FMS PowerPrep™ SPE extraction system in combination with the PowerVap™ concentrator to extract PAHs from water. Read More		 Extraction of Bath Salts (substituted cathinones) From Human Urine Using ISOLUTE® SLE+ Columns prior to GC-MS Analysis
Rhys Jones, Biotage AB
This application note describes the supported liquid extraction clean-up of a range of substituted cathinones from urine prior to quantitative GC-MS analysis.  Analyte recoveries achieved using this method to extract bath salts from urine  ranged from 87-99% with RSDs  below 10% for all analytes. Read More
 
HPCL
Metabolic Profiling of Yeast Sterols Using the Agilent 7200 Series GC/Q-TOF System
Manhong Wu, Robert St. Onge, Sundari Suresh, Ronal Davis, and Gary Peltz, School of Medicine and Biochemistry, Genome Center, Stanford University; and Sofia Aronova and Stephan Baumann, Agilent Technologies, Inc.
The 7200 GC/QTOF and Mass Profiler Professional (MPP) were used to determine the enzymatic targets of putative antifungal drugs. This was done by using high resolution and accurate mass MS and MS-MS data to determine the relative levels of ergosterol biosynthesis intermediates. Read More		 Analysis of PNA in Jet Fuel by Flow Modulated GCxGC
Rob de Jong, PAC
AC Analytical Controls developed a robust solution that provides quantitative insight into the group type composition of jet fuels. The solution, based on flow modulated GCxGC uses advanced AC Flow modulation, simplifying hardware and avoiding the need for large volumes of liquid coolants normally required for cryogenic modulation. AC Flow Modulation yields better peak resolution and requires less operator intervention, making it suitable for routine lab operation. This application note discusses this new technique, which determines total paraffins, total naphtenes, and total aromatics (PNA) for jet fuel. Read More

GC-MS/MS Analysis of OCPs, PAHs, and PCBs in Environmental Samples
Inge de Dobbeleer, Joachim Gummersbach, Hans-Joachim Huebschmann, Anton Mayer, Paul Silcock, Thermo Fisher Scientific
A high performance, highly productive analysis of OCPs, PAHs, and PCBs in environmental samples is conducted through a consolidated GC-MS/MS method.  Smart software tools are integrated into the method development and analysis workflow to minimize the time needed to implement and maintain the methodology in routine. Read More
 
GPC
Simultaneous Quantitative and Qualitative Measurements in a Single Workflow
Don Richards, Bruker Daltonics
This application note describes an investigation using a qTOF instrument to obtain clearance data, matabolite identification, structure elucidation, and metabolite profiles from P450 microsomal incubations at a drug concentration of 1 µm from a single sample set. Read More
 
GPC
Content uniformity test of Coenzyme Q10
CAMAG Laboratory
Coenzyme Q10 is assumed to enhance the immune system and to work as an antioxidant protecting against free radicals that can damage cells. As dietary supplement it is mostly sold in soft gelatin capsules. For quality control of such products the content uniformity test (CUT) and the assays are of high interest. This method is suitable for the quantification of coenzyme Q10 in gel capsules. Read More		 Structural Confirmation of Polymeric Material
Kirsten Craven, Michael McCullagh, Pascal Gerbaux, Julien De Winter, Waters Corporation
Structural Confirmation of Polymeric Material Using MS/MS and Fragmentation Mechanisms. This application note uses polylactide to demonstrate how MS/MS fragmentation patterns can be used to help determine the backbone architecture of a polymer. Read More

UPLC Screening to Preparative HPLC
Mia Summers and Kenneth J. Fountain, Waters Corporation
This application note demonstrates a rapid UPLC screening protocol using high and low pH on a charged-surface hybrid (CSH) column and subsequent scale-up to an efficient preparative HPLC purification using focused gradients. The CSH column is chosen for its improved peak shape for basic compounds, wide pH range capability, and retention time stability with routine pH switching. The use of the same column stationary phase on the preparative scale maintains the separation selectivity from the UPLC screening run, alleviating the need for method redevelopment. Read More
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