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Featured Application Note:
Simplifying Complex Multi-Residue Pesticide GC-MS/MS Methodology
David Steiniger, Juan Carmona, Stacy Crain, Paul Silcock, and Jason Cole,
Thermo Fisher Scientific
Ease the implementation and management of multi-residue pesticide methodologies with a comprehensive solution that enables quick and easy method development regardless of method complexity and experience level of the user to eliminate days, if not weeks, from normal development time. Read More |
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Simultaneous Quantitative and Qualitative Drug Metabolism Investigations
Don Richards, Bruker Daltonics
The ability to simultaneously collect quantitative and qualitative information from a DMPK-analysis has the potential to significantly increase productivity in pharmaceutical drug discovery and development. This work describes an investigation using the latest quadrupole-time-of-flight mass spectrometer to obtain clearance data, metabolite identification, structure elucidation, and metabolite profiles from Cytochrome P450 microsomal incubations in a single sample set. Read More |
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SEC-MALS Analysis of Polycarbonate Urethanes
Jason Todd, Wyatt Technology
Analysis of polycarbonate urethanes (PCUs) are a class of biocompatible polyurethanes that show great promise for use in orthopaedic implants, such as artificial hips. The molar mass distribution of a PCU is of critical importance for its performance in medical device applications and the absolute distributions of PCUs can only be obtained reliably from light scattering analysis. Read More |
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Branched Copolymer Characterization by Triple Detection GPC
Malvern Instruments
Detailed description of the analysis and characterization of four different synthetic copolymers is presented in this application note. A triple detection GPC system, equipped with light scattering and viscometer detectors, was used to calculate a range of parameters for a number of branched polymers. These included the molecular weight and polydispersity, hydrodynamic radius, and molecular weight dependence of the viscosity, branching number, and branching frequency. Read More |
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Analysis of Beer by Comprehensive 2D-LC
Edgar Naegele and Keiko Hayashi, Agilent Technologies
This application note demonstrates the use of two-dimensional liquid chromatography for the separation of complex compounds mixtures inherent in commercially available beer samples. Read More |
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Characterization of Glycosylation in the Fc Region of Therapeutic Recombinant Monoclonal Antibody
James Martosella, Phu T. Duong, and Alex Zhu, Agilent Technologies, Inc.
In this note, we describe a fast and efficient liquid chromatography time-of-flight mass spectrometry approach to characterize an IgG derived from CHO cells. An Agilent Poroshell 300SB-C3, 5 µm superficially porous IgG1 column was used to obtain accurate glycoform masses of an intact IgG1, with a subsequent analysis of papain-digested IgG1 to obtain site-specific glycosylation profile information of the Fc region for further characterization of IgG1 heterogeneity. Additionally, a subsequent mAb-glyco chip LC–MS analysis was used to build a glycan accurate mass database for additional validation of the glycans identified in the TOF mass analysis. Read More |
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Automated Solid Phase Extraction (SPE) and Concentration of Organochlorine Pesticides in Water
Phillip Bassignani, FMS, Inc.
Organochlorine pesticides are man-made organic chemicals with a history of wide spread use throughout the world. Tending to be very persistent in the environment, they have found their way into sediments and drinking water supplies posing serious health risks. The automated solid phase extraction and concentration method described allows for rapid, reproducible extractions using minimal solvent volume that produces consistent results. Read More |
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Fast Extraction of Metanephrine and Normetanephrine from Urine Using EVOLUTE EXPRESS CX SPE Plates
Biotage AB, Sweden
A new solid phase extraction technology has been developed to speed up sample preparation by eliminating steps in the extraction workflow. This application note describes the extraction of metanephrine and normetanephrine from synthetic urine using this 'load-wash-elute' approach with EVOLUTE EXPRESS CX 96-well SPE plates. Recoveries exceeding 80% and excellent linearity over the range 10-70 ng/mL are achieved. Read More |
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Rapid Automated Screening of Extractable Compounds in Materials for Food Packaging, Medical, or Technical Purposes (Extractable Compounds in Materials)
Oliver Lerch, GERSTEL
Two automated methods for screening of extractable compounds from materials for food packaging, medical, or technical purposes are described based on automated liquid extraction and thermal desorption, respectively. Both methods are suitable for gaining an overview of the quality and emission potential of a material to determine its usefulness as a packaging material. The methods deliver comparable qualitative results. Read More |
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Providing a Universal, One-step Alternative to Liquid-Liquid Extraction in Bioanalysis
Jessalynn P. Wheaton, Erin E. Chambers, and Kenneth J. Fountain, Waters Corporation
The Ostro sample preparation plate and a generic liquid-liquid extraction (LLE) method were compared for the extraction of a diverse group of pharmaceutical drugs. With no method development, analyte recovery was high, >75%, for polar and nonpolar, acidic, and basic analytes alike, and 95% of phospholipids were removed relative to LLE. Ostro extraction of 96 samples was achieved in half the time relative to 96-well LLE and in less than 1/10th the time if LLE was performed in individual tubes. Read More |
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