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Transfer the USP Method for Cephradine from a Traditional 5 µm Column to Poroshell 120
Rongjie Fu, Agilent Technologies
A method for cephradine was run on a traditional 5 µm column according to the United States Pharmacopeia (USP) assay for this drug. The method was transferred to a superficially porous Agilent Poroshell 120 ECJ-C18 Column, which allowed for significant time savings within the guidelines in USP Chapter 621. The system requirements were all met with the Poroshell 120 ECj-C18 Column. Read More |
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Analysis of Glyphosate and AMPA in Drinking Water Using Online SPE
Bettina Schuhn and Neil Cullum, Agilent Technologies
This application note describes a robust and easy-to-use method for the determination of glyphosate and AMPA in water using online SPE. Read More |
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Simultaneous Quantification of Neurotransmitters in Human Cerebrospinal Fluid (CSF)
Mary E. Lame, Erin E. Chambers, Kenneth J. Fountain, Waters Corporation
The method demonstrates the simultaneous quantification of ACh, HA, and their metabolites in human CSF in a 96-well format. This application uses a single-step sample preparation followed by HILIC UPLC/MS/MS analysis. The high efficiency CORTECS UPLC HILIC column provided resolution from endogenous matrices, increased analyte sensitivity, and a short analysis time of 2.5min, thereby resulting in a fast, sensitive, selective, and accurate method that meets the demands of high-throughput bioanalytical drug discovery. Read More |
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RI Detector for UHPLC
Dan Some, Wyatt Technology
The first refractive index detector for use with UHPLC. Read More |
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Pressurized Liquid Extraction and Direct to Vial Concentration of Organochlorine Pesticides in Soil
Phillip Bassignani, Fluid Management Systems
Pesticides are classes of chemicals that are used to treat or control outbreaks of pests, especially insects. Food production facilities as well as safety and health organizations heavily rely on pesticides to increase food yields or control diseases spread by insects. Some pesticides have proven hazardous to other animals or the environment and have either been restricted or banned. The need to monitor food products for pesticides is essential as more pesticides are being discovered to have adverse effects. One class of commonly used pesticides are chlorinated pesticides. These compounds are commonly detected using EPA Method 8081. This method determines the concentrations of various organochlorine pesticides in extracts from solid and liquid matrices, including food products, by GC with electron capture detectors (ECD). Read More |
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Extraction of Acrylamide from Coffee Using ISOLUTE SLE+
Alan Edgington, Biotage AB, Sweden
The method outlined in this application note demonstrates a simple, rapid, and robust method for the extraction of acrylamide from ground coffee and instant coffee. Detection limits of 1 ng/mL are achieved from this challenging matrix. Read More |
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Determination of Chlorophenoxyacetic Acid and Other Acidic Herbicides Using a QuEChERS Sample Preparation Approach and LC–MS-MS Analysis
Brian Kinsella, UCT, Inc.
2,4-Dichlorophenoxyacetic acid (2,4-D) is a common herbicide used in the control of broadleaf weeds. Chlorophenoxyacetic acid herbicides, such as 2,4-D, along with other acidic pesticides can be extracted from fruits and vegetables using the QuEChERS approach outlined in this application note. LC–MS-MS is used for detection and quantitation. Read More |
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Mud Logging – Rapid Analyses of Well Gases with an Agilent Micro GC
Remko van Loon, Agilent Technologies
Analytical testing of drilling fluid samples using the Agilent Micro GC has proven to be an excellent technique for analyzing individual hydrocarbon gases to assist with lithology reports for the mud logging field. The instrument's small form factor and low consumption of operating gas provides an ideal solution to incorporate into drilling operations. Read More |
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Semivolatile Analysis with Specially Designed Agilent J&W DB-UI 8270D Ultra Inert GC Columns
Ken Lynam, Agilent Technologies
Excellent peak shapes and chromatographic performance for challenging semivolatile analytes are shown on 0.25 mm and 0.18 mm i.d. Agilent J&W DB-UI 8270D columns. The utility of using a 29-semivolatile analyzer mix is demonstrated in less than 16 min on a 20 m x 0.18 mm, 0.36 µm DB-UI 8270D capillary GC column. The use of Agilent Ultra Inert liners with wool is also demonstrated on a high efficiency GC (0.18 mm i.d.) column. Read More |
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Molecular Weight Measurement of an Antibody by SEC-MALS
Malvern Instruments
In this application note, a purified polyclonal antibody (IgG) is separated using SEC and characterized using the Viscotek SEC-MALS 20. The molecular weights of the IgG monomer and dimer were successfully measured, as well as the molecular weight and radius of gyration (Rg) of the IgG aggregates. Read More |
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Radio ion chromatography
M. Espinosa1, J. Chesa-Jiménez2, B. Galliker3, A. Steinbach3, and A. Wille3
1Gomensoro S.A., 2 Instituto Tecnológico PET, 3 Metrohm International Headquarters
This article presents a likewise rugged and versatile multichannel radio ion chromatography (IC) system that controls the production of the radionuclide [18F]fluoride (precursor) and the two radiotracers synthesized from it, [18F]fluorodeoxyglucose and [18F]fluorocholine, in accordance to pharmacopoeial regulations. Read More |
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Method Development for Impurity Analysis Using ACQUITY UPLC H-Class System with an ACQUTY QDa Detector
Margaret Maziarz, Waters Corporation
The ACQUITY QDa Detector aids in the development of efficient and robust screening methods by minimizing the need for standard runs to confirm the identity of peaks by retention time. Read More |
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