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Featured Application Note:
Accurate Compound Measurement in Metabolomics: Isotope Ratio Outlier Analysis (IROA) protocol
Kevin J. McHale, Mark Szewc, Gregory S. Stupp, Chaevien Clendinen, Ramadan Ajredini, Arthur S. Edison, and Chris Beecher, Thermo Fisher Scientific
The combination of IROA and HR/AM LC–MS–MS enables metabolites to be distinguished in an untargeted manner. Download this application note and learn more about metabolite quantitation using IROA and the Thermo Scientific™
Q Exactive™ mass spectrometer. Read More |
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Branching Revealed by SEC-Multi Angle Light Scattering (MALS)
Stepan Podzimek, Wyatt Technology
The abnormal elution behavior of branched macromolecules in size exclusion chromatography (SEC) is explained and the data acquired by SEC-MALS are compared with those obtained by asymmetric flow field flow fractionation (A4F). Read More |
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How to Gain More Information About the Conjugation State of Your Polymer
Malvern Instruments
A photodiode array detector (PDA) used in conjunction with a gel permeation chromatography (GPC) system adds new insight into the properties of polymers, that can assist with designing the optimum polymer for applications such as an OLED emitter. This collaborative application note uses three different examples to highlight the benefits of adding a PDA to your GPC system. Read More |
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The Fully Automated Solid Phase Extraction of Aqueous Samples by Method EPA 8270D
Philip Bassignani, FMS,Inc.
EPA 8270D calls for the extraction for analysis of semi-volatile analytes in various matrices. Target analytes mentioned in the method cover a wide range of compound classes resulting in reporting lists that often approach 100 compounds. In aqueous samples, the combination of compound groups such as phenols, analines, PAHs, phthalates, explosives, pesticides, n-nitro-soamines, and others results in a tedious process of multiple LLE (liquid-liquid extractions) with multiple pH adjustments to extract all the analytes. Read More |
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Extraction of Basic Drugs from Urine with no Dry Down Step Using EVOLUTE EXPRESS CX SPE Prior to LC–MS–MS
Victor Vandell and Frank Kero, Biotage AB, Sweden
A novel method using EVOLUTE EXPRESS that follows a LOAD-WASH-ELUTE-ANALYZE strategy. Basic drugs are fortified into urine and extracted in 3 easy steps using EVOLUTE EXPRESS CX solid-phase extraction in a 96-well plate format. Read More |
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Determination of Carboxy-THC (THCA) in Urine Samples Using Solid-Phase Extraction and GC–MS
Jeff Hackett, UCT, LLC
This methodology details the isolation of Carboxy-THC (THCA) from urine samples using solid-phase extraction on Clean Screen THC columns. After rinsing and drying the sorbent, the THCA was eluted using a solution of acidified ethyl acetate/hexane. The eluate was dried and derivatized using MSTFA prior to analysis by GC–MS. Read More |
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interaction and a C-18 column on the Agilent 1290 Infinity Quaternary Method Development solution.