|
|
 |
Effective Sample Preparation and Rapid LC–MS–MS Analysis of a Large Pain Panel Screen Using Strata–X–Drug B Solid Phase Extraction (SPE) and Kinetex Phenyl-Hexyl Core-Shell Technology HPLC/UHPLC Columns
Sky Countryman, Shahana Huq, and Erica Pike, Phenomenex
Using a single SPE method on a specialized sorbent, 41 pain management drugs from several drug classes were isolated from urine samples and analyzed by LC–MS-MS in under 5 min. Read More |
 |
Automated, High-Throughput Analysis of Benzodiazepines from Urine and Plasma Using Strata-X 96-Well Plate Solid Phase Extraction (SPE) and Kinetex Core-Shell Technology HPLC/UHPLC Columns
Shahana Huq, Seyed Sadjadi, Sky Countryman, and Erica Pike, Phenomenex
Eight benzodiazepines were extracted from both urine and plasma using an automated SPE procedure followed by a rapid (<3 min) LC–MS-MS analysis, providing the opportunity to substantially increase sample throughput and simultaneously decrease costs per sample.
Read More |
|
|
|
Simple and Fast Quantitation of Nicotinic Acid and Nicotinamide in Human Plasma by Applying Impact Protein Precipitation Plate Technology with Gemini 3 µm C18 HPLC Columns
Shuguang Li and Erica Pike, Phenomenex
Nicotinic acid and nicotinamide were extracted from human plasma by performing a rapid protein precipitation followed by HPLC analysis, which produced excellent chromatographic resolutions, sensitivity, and high peak capacities at an LOD of 2 ng/mL. Read More |
|
Analyzing Testosterone in Human Serum by UHPLC Using High Efficiency Kinetex® 1.7 µm C18 Core-Shell Columns
Seyed Sadjadi and Jeff Layne, Phenomenex
Testosterone is extracted from human serum using a strong anion-exchange polymeric SPE sorbent, followed by a rapid LC–MS-MS analysis using a high efficiency UHPLC column. Read More |
|
|
|
|
Rapid LC–MS-MS Analysis of Digoxin and Digitoxin in Plasma Using Strata™-X SPE Cartridges and Kinetex 2.6 µm C8 Core-Shell HPLC/UHPLC Columns
Matthew Trass, Jeff Layne, and Phil Koerner, Phenomenex
Digoxin and digitoxin are effectively extracted and concentrated from plasma using a polymeric solid phase extraction (SPE) sorbent and are further analyzed by a rapid LC–MS-MS analysis over a concentration range of 0.25 to 10 ng/mL. Read More |
|
Rapid Improvements for LC–MS-MS Analysis Without Additional Method Development Using Phree™ Phospholipid Removal Plates
Dr. Stuart Kushon and Erica Pike, Phenomenex
Protein precipitation is compared to a phospholipid removal product, Phree Phospholipid Removal Plates, to assess the cleanup capabilities of each technique. By measuring for total phospholipids using the 184->184 mass transition, LC–MS MS analysis indicates that Phree Phospholipid Removal Plates result in significantly cleaner samples, reduced ion suppression, and extended HPLC/UHPLC column lifetime. Read More |
|
|
| |
 |
Rapid Separation of 25-OH-Vitamin D3 and 3-epi-25-OH-Vitamin D3 in Human Serum Under RP-LC Conditions and Tandem Mass Spectrometry Detection
Seyed Sadjadi and Jeff Layne, Phenomenex
A rapid LC–MS-MS analysis is performed using a high efficiency media that allowed for separation of the monohydroxy vitamin D3 isomers as well as separation of the 3-epi-25 monohydroxy epimer. Read More |
|
Rapid, Automated Extraction and LC–MS-MS Analysis of Tricyclic Antidepressants from Plasma Using Strata-X-Drug B SPE and a Kinetex Core-Shell HPLC/UHPLC Column
Shahana Huq, Seyed Sadjadi, and Erica Pike, Phenomenex
Using an automated SPE method that requires no condition or equilibration steps, time and solvent are saved during the analysis of nine tricyclic antidepressant (TCA) compounds from human plasma. Coupled to a rapid LC–MS-MS method using a Kinetex Core-Shell HPLC/UHPLC column, this method can instantly provide time and solvent savings to high throughput laboratories. Read More |
|
A Simplified and Streamlined Approach to Solid Phase Extraction (SPE) Using Strata-X-Drug B for SAMHSA’s Low Cutoff Drugs of Abuse Panel
Michael Rummel, Matthew Trass, Seyed Sadjadi, and Erica Pike, Phenomenex
Utilizing a single SPE sorbent and only three extraction methods, 11 drugs from six different substance abuse and mental health services administration (SAMHSA) regulated drug classes were effectively extracted and analyzed by LC–MS and GC–MS. Read More
|
|
|
| |
|
|
|
