January 2013
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Biopharm
Analysis of 2-aminobenzamide Labeled Dextran Ladder on a Solid Core nanoLC Column
Joanna Freeke and Valeria Barattini, Thermo Fisher Scientific
This application note demonstrates the analysis of a dextran ladder labeled with 2-aminobenzamide by HILIC chromatography. The separation is carried out with a Thermo Scientific™ Accucore™ 150-Amide-HILIC (150 Å pore diameter solid core) nanoLC column. The method is simple, robust, and features excellent separations in conditions compatible with MS detection.
 
Analysis of 2-aminobenzamide Labeled Dextran Ladder on a Solid Core HPLC Column
Valeria Barattini, Kim Phipps, and Joanna Freeke, Thermo Fisher Scientific
This application note demonstrates the analysis of a dextran ladder labeled with a fluorescent tag 2-aminobenzamide by HILIC chromatography. The separation is carried out with a Thermo Scientific™ Accucore™ 150-Amide-HILIC (150 Å pore diameter solid core) HPLC column. The method is simple, robust, and features excellent separations at backpressures compatible with conventional HPLC systems.
 
Analysis of Estrogens Using a Solid Core HPLC Column
Jamil Ali, Thermo Fisher Scientific
This application note demonstrates the use of the Thermo Scientific™ Accucore™ Phenyl-X HPLC column for the analysis of aromatic steroids. When compared with a C18 column, the Accucore Phenyl-X HPLC column provides high aromatic selectivity, good hydrophobic retention, and unique, complementary selectivity.

Pharma
Separation of a Mixture of Vitamin K Isomers Using a Solid Core HPLC Column at Sub-Ambient Temperature
Derek Hillbeck, Thermo Fisher Scientific
A high speed, high resolution separation of vitamin K1 and K2 was achieved on a Thermo Scientific™ Accucore™ C30 column. The high shape selectivity of this phase enabled the separation of the cis/trans isomers of vitamin K1. The separation of the isomers is temperature dependent and is optimum at around 15 °C.
 
Analysis of 3 Nonsteroidal Anti-Inflammatory Drugs Using a Solid Core HPLC Column
Joanne Jones, Thermo Fisher Scientific
This application note demonstrates the selectivity of the Thermo Scientific™ Accucore™ Polar Premium HPLC column for the fast analysis of three nonsteroidal anti-inflammatory drugs.
 
Rapid Separation of Analgesic Compounds Using a Solid Core HPLC Column
Derek Hillbeck, Thermo Fisher Scientific
This application note demonstrates the use of a Thermo Scientific™ Accucore™ Urea-HILIC column for the rapid analysis of the analgesic compounds aspirin, acetaminophen, and salicylic acid.