LCGC Europe Application Note Alert
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Featured Application Note:
Solid-Phase Extraction for the Analysis of Drugs of Abuse in Urine
Many drugs of abuse (DOA) — whether natural or synthetic — are rapidly excreted from the human body. However, some can persist for extended periods. Screening for traces of DOAs has become commonplace by analyzing human urine with liquid chromatography–mass spectrometry (LC–MS) or gas chromatography–mass spectrometry (GC–MS) through derivitization of the parent compounds. Solid-phase extraction (SPE) provides an ideal extraction media for DOA compounds, as it can be performed with small sample amounts with rapid turnaround.
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Analysis of a Fixed-Dose Combination Drug Using the Agilent 1290 Infinity II ELSD
This application note demonstrates the gain in performance and efficiency in sample throughput when using an Agilent 1290 Infinity II ELSD in the analysis of a fixed-dose combination drug.
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Catecholamine Analysis by LC–MS–MS: Method Development
This poster describes method optimization of catecholamine analysis by liquid chromatography tandem mass spectrometry (LC–MS–MS) extracting the analytes dopamine, epinephrine, and norepinephrine. It was shown that the polymer-based EVOLUTE® EXPRESS WCX 10 mg 96 well plate can be used to extract the analytes from human plasma in a highly sensitive linear assay. Optimization was performed for a number of different steps including washing, extraction, elution, and mobile phase composition.
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LC–MS–MS Method for 89 Banned or Controlled Drugs in the Horse Racing Industry
The world of equine drug testing is constantly evolving. New regulations regarding banned and controlled substances can be found throughout North America, Europe, and Australia. The need for a reliable LC–MS–MS method for the determination and separation of a wide variety of drug classes is critical. The unique polyaromatic phase of the Selectra® DA HPLC column allows for efficient analysis and baseline separation of multiple drug classes at once, including several complex isobaric compounds.
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Absolute Molar Mass in UHPLC via the µDAWN and UT-rEX
Ultrahigh-performance liquid chromatography (UHPLC) enables fast, efficient separation of biomolecular samples. Compared to standard SEC, UHPLC–SEC provides improved resolution, higher throughput, less solvent, and smaller sample consumption for analysis of precious biological samples.
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Type I Ultrapure Water Crucial for HPLC and UHPLC
High performance liquid chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify compounds with a wide range of differing properties. The need to ensure that standards, organic solvents and HPLC mobile phases are of the highest purity is widely recognised, yet water quality, which may have a major impact on chromatographic performance, is frequently taken for granted. For Ultrahigh-performance liquid chromatography (UHPLC), a technique using sub-2 µm particles and high pressures to enhance analytical speed.
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Veterinary Drug Residues in Food
The permitted presence and levels of veterinary drug residues in food of animal origin are legislated in the EU, and can vary depending on the drug in question. This MicroLC method using a YMC-Triart C18 capillary column easily fulfils the requirements of the current EU legislation. Furthermore, the microLC–MS–MS approach has the additional advantage of being a potential drug residue screen where different residues can be detected by a single method.
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Chiral Separation of the Fungicide Metalaxyl
Metalaxyl is a fungicide used to fight plant diseases in agriculture. Metalaxyl has two enantiomers, of which (R)-Metalaxyl has a higher activity. The valid determination and quantification of Metalaxyl residues is very important for monitoring food, soils and ground water. CHIRAL ART Cellulose-SB separates the enantiomers of Metalaxyl easily.
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Separation of Structural Analogues: Vitamin D2 and D3
Separations of structurally similar compounds such as vitamin D2 and vitamin D3 are very challenging. Standard C18 columns such as YMC-Triart C18 are not able to fully separate the two vitamins. A very hydrophobic phase with a higher density and therefore amount of C18 chains is required. YMC-Triart C18 ExRS is able to separate these two.
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Fast Analysis of Isoflavonoids in Food
Soy is the most important source of vegetable oil worldwide. It contains a high level of isoflavonoids that can have a significant positive effect on hormonal balance. The following method allows for a fast and robust separation of isoflavonoids and facilitates the analysis of these food ingredients.
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Usability Study of a New HPLC, a New Tandem MS and a New Data Processing Software
This usability study evaluated a new two-channel HPLC system, a new tandem MS and data processing software for general clinical use, focusing on the areas of stability, robustness and time efficiency.
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Instant Sensitivity Gains Using µElution Solid-Phase Extraction (SPE)
Solid-phase extraction (SPE) is an excellent cleanup solution for bioanalytical samples because it is selective, reproducible, and results in ultra-clean and concentrated samples. While the technique is effective, traditional formats such as 10 mg 96-well plates or tubes pose challenges for small or limited sample volumes and can result in dilute eluents if the sample is not dried down and reconstituted after the extraction procedure. This dry down step can require 30 or more minutes, adding a significant amount of time to the procedure. To overcome these challenges, the µElution 96-well SPE plate format was developed. The µElution SPE plates contain significantly less sorbent as compared to a traditional 10 mg 96-well SPE plate, allowing analysts to elutein volumes as low as 25 µL. These low elution volumes result in ultra-concentrated samples that do not need to be dried down. In this study, we compared the µElution 96-well SPE plate to a traditional 10 mg 96-well SPE plate to determine the amount of sensitivity gain that can be achieved by moving a 10 mg SPE method to the µElution format.
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Detection Limits for GC–TOF MS of Organotins Using Select-eV Variable-Energy Electron Ionization
This application note demonstrates how the enhanced sensitivity and selectivity of the BenchTOF time-of-flight mass spectrometer with Select-eV variable-energy electron ionization can provide ultra trace-level quantitation of organotins in complex environmental extracts. We focus on how Select-eV soft ionization offers particular advantages in the analysis of organotin species, which undergo extensive fragmentation at conventional 70 eV ionization energies, by producing simplified spectra with enhanced diagnostic ions.
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