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Photo Credit: GIPhotoStock/Getty Images |
Trends in Sample Preparation
The results obtained from a new survey on sample preparation techniques were compared with the results of previous surveys from 1991 to March 2013. The survey investigated trends in technologies currently being used, sample loads, sample sizes, automation, the use of solid-phase extraction (SPE) devices (cartridges, disks, plates, tips), SPE chemistries, selection criteria, and problems encountered. |
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The LCGC Blog: Practical HPLC Method Development Screening
Tony Taylor considers the other important aspects of “screening” in HPLC. Discussing the importance of the mobile phase composition, gradient parameters, and flow rate when developing new HPLC methods. Offering tips and advice to ensure good efficiency, peak shape, and practice. |
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Photo Credit: Michael Leggero/Getty Images |
Analyzing Antarctic Ice Cores Using Capillary Ion Chromatography
Ice cores contain an abundance of information about climate and the changes it is undergoing. Brett Paull and Estrella Sanz Rodriguez from the Australian Centre for Research on Separation Science (ACROSS) at the University of Tasmania, Hobart, Australia, spoke to Kate Mosford of The Column about their work on the analysis of Antarctic ice cores and the important role of capillary ion chromatography (cap-IC) in this area of research. |
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Photo Credit: Donald Jusa/Getty Images |
Compositional Analysis of Honeybee Venom for Proteomics
Research into honeybee venom is being performed to identify active proteins acting as allergens and toxins. Researchers combined LC–MALDI-TOF/TOF-MS–MS, LC–ESI-QTOF-MS–MS, and a combinatorial peptide ligand library enrichment method to detect 269 proteins. |
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Peak Purity Algorithms Using Diode Array Detectors
In an ideal chromatographic separation, all sample components would be isolated from each other and detected as fully resolved peaks. However, it is not uncommon to encounter some degree of overlap. In extreme cases, what appears to be a single peak contains in fact two or more coeluting components. This article discusses how to check the purity of such peaks in order to correctly interpret the results of the analysis. |
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