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14 April 2016 |
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HPLC 2016 Previews—Trends In Separation Science, Part 1: Why Effective HPLC Separations Are Critical for the Analysis of High-Complexity Systems
In proteomics studies, proteins are digested into hundreds of thousands of peptides, thus creating very large and complicated mixtures. Simultaneous electrospray ionization of these complex mixtures, however, results in suppression of ion formation. Therefore, it is essential to have effective chromatographic methods to separate the peptides before analysis with mass spectrometry, to relieve ion suppression and to allow the mass spectrometer sufficient time to collect tandem mass spectra of peptide ions. The challenges involved in developing such separations are great, however.
This is the first of a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24. |
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HPLC 2016 Previews—Trends In Separation Science, Part 2: New Materials for UHPLC Analysis of Monoclonal Antibody Drugs and Antibody–Drug Conjugates
Separations of intact proteins play many roles in drug discovery and development. A variety of separation techniques are used, from immunoprecipitation for study of a single protein of interest, through various types of column chromatography for detecting a handful of proteins at once, all the way to proteomics for studying hundreds to thousands of proteins. What all of these techniques and applications have in common is that the power of protein separations is limited by the fact that proteins are large, slowly diffusing, sticky molecules. This article discusses various chromatographic approaches to addressing this challenge.
This is the second in a series of articles exploring topics that will be addressed at the HPLC 2016 conference in San Francisco, from June 19 to 24. |
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| Application Note |
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 Photo Credit: Michael Leggero/Getty Images |
Analyzing Antarctic Ice Cores Using Capillary Ion Chromatography
Ice cores contain an abundance of information about climate and the changes it is undergoing. Brett Paull and Estrella Sanz Rodriguez from the Australian Centre for Research on Separation Science (ACROSS) at the University of Tasmania, Hobart, Australia, spoke to Kate Mosford of The Column about their work on the analysis of Antarctic ice cores and the important role of capillary ion chromatography (cap-IC) in this area of research. |
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