Featured Application Note A Simple HILIC Method Development Protocol This Knowledge Note describes the nature of HILIC analyses and provides a simplified and systematic protocol for HILIC method development using three HILIC phases Sample Prep Fully Automated Solid-Phase Extraction of Base, Neutral, and Acid Semivolatiles in Industrial Wastewater The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process. Effective Lipid Cleanup in LC–MS/MS Analysis of Drugs in Human Serum A sample prep method using PPT followed by Agilent Captiva Enhanced Matrix Removal—Lipid cleanup for the quantitative determination of nine drug compounds. All-in-One Analysis of Pesticide Residues, Mycotoxins, and Potency in Cannabis A QuEChERS method for the simultaneous analysis of cannabis for 47 pesticides, 5 mycotoxins, and 3 cannabinoids, with sample purification using SpinFiltr™.   HPLC Chiral Multicolumn Method Development with SFC The Agilent 1260 Infinity II Analytical SFC system performed automated method development, screening four chiral columns under different conditions. Mass-Directed Purification of Piperine from Piper nigrum Gilson has developed a purification method for piperine using centrifugal partition chromatography (CPC) with mass-directed fraction collection. A Rugged 4-Min Method for High-Throughput Labs The dilute-and-shoot EtG and EtS LC–MS/MS analysis developed here using the novel Raptor EtG/EtS column easily resolves EtG and EtS from matrix interferences.   GC Going Beyond EPA Method TO-15: Analysis of Trace-Level “Air Toxics” at High Humidity GC–MS analysis of trace-level “air toxics” in humidified canister air, using Markes’ groundbreaking cryogen-free Dry-Focus3 preconcentration technology is described. Residual Solvents in Pharmaceuticals by USP Chapter 467 This application note discusses how USP chapter 467 method classifies residual solvents in pharmaceuticals into three classes, class 1, class 2A, and class 2B.   Hyphenated Metabolomics Study with HILIC–UHPLC–MS This application note details a nontargeted metabolomics study on cell extracts that was performed with a HILIC–UHPLC–MS setup.   GPC PLGA Molecular Weight and Conformation Analysis The problems of characterizing PLGA can be overcome by using a triple-detection GPC system comprising multi-angle light scattering, viscometry, and RI detectors.