Top 16 Articles of 2016

As another year of chromatographic excellence passes into history the LCGC editorial team celebrates some of the highlights of last year by selecting the top 16 articles of 2016.

1. A Universal Reversed-Phase HPLC Method for Pharmaceutical Analysis

This universal method was fast (2 min) and provided reasonable peak capacity and excellent peak shapes for many new chemical entities. Read more

2. How Does It Work? Part 1: Pumps

If you understand how pumps operate, it is easier to solve problems. “LC Troubleshooting” Editor John W. Dolan explains what you need to know. Read more

3. Glycosylation in mAb Therapeutic Products: Analytical Characterization and Impact of Process

This instalment of the “Biotechnology Today” column reviews best practices and recent advances in characterizing glycosylation. Read more

Reduce LC-MS Equilibration Time Costs

TARGA^® RPC capillary columns, capable of equilibrating up to 10x faster in 100% water, speed the analysis of polar glycans, nucleotides and peptides saving LC-MS instrument time. Have us custom pack your media or try our TARGA C18 capillary columns to compare! The Nest Group, Inc. - "Not Just Columns ... Answers!"

4. Trends in Sample Preparation

Doug Raynie presents a comparison of the results from a new survey on sample preparation techniques to those of previous surveys, from 1991 to 2013, revealing some interesting trends. Read more

5. Analysis of Nicotine Alkaloids and Impurities in Liquids for e-Cigarettes by LC–MS, GC–MS, and ICP-MS

Various analytical MS methods were developed to investigate the chemical composition of liquids used in electronic cigarettes and characterize their quality. Read more

6. Generic UHPLC Method for the Simultaneous Analysis of Compounds with a Wide Range of Polarities

The generic method described here involves a UHPLC system equipped with two external switching valves to connect HILIC and reversed-phase LC columns in series for the sequential analysis of polar and apolar compounds, for use in fields such as environmental analysis, biomarker discovery, and proteomics. Read more

7. Effects of Column Inner Diameter and Packed Bed Heterogeneities on Chromatographic Performance

This instalment of “Column Watch” looks at the sources of band broadening in small-diameter HPLC columns, with an emphasis on eddy dispersion. Read more

8. Statistics for Analysts Who Hate Statistics, Part I: Collect and Examine Your Data

This first instalment in a new series of very short tutorials explains, in the simplest manner, how statistics can be both useful and accessible, even for chromatographers who normally find statistics difficult. Read more

9. Practical HPLC Method Development Screening

A frequently asked question about the other important aspects of “screening” in HPLC, including mobile phase composition, gradient parameters, and flow rate, inspired the theme of this instalment of The LCGC Blog. Read more

10. Simplifying Mass Spectrometry Through New Ionization Technology: Application to Drugs and Clinical Analyses

A newly discovered method, matrix-assisted ionization (MAI), offers a new ionization approach for problems not readily addressed by current technologies, as well as simplicity, sensitivity, and cost effectiveness. Read more

11. UHPLC Instrument Variations and Approaches to Ease the Method Transfer Process

This study determined the typical inter-instrument variations in dwell volume, extracolumn dispersion, and mixing efficiency, and assessed techniques to account for the variations. Read more

12. An Indisputable Case of Matrix Effects in Blood Alcohol Determinations

While reviewing a blood alcohol investigation, Kevin A. Schug discovered major method validation protocol deficiencies. Matrix effects may have resulted in blood alcohol concentrations being over-estimated, resulting in a potentially significant injustice. Read more

13. How Can LIMS Help Ensure Data Integrity?

In the world of data integrity, the focus is typically on the data and the numbers. How can technology, such as LIMS, help to ensure data integrity? This “Questions of Quality” instalment from Bob McDowall provides explores this question. Read more

14. Maximization of Selectivity in Reversed-Phase Liquid Chromatographic Method Development Strategies

This paper aims to assist chromatographers in producing highly efficiency method development strategies for reversed-phase LC separations in a relatively short timeframe. Read more

15. Quality by Design: A Tool for Separation Method Development in Pharmaceutical Laboratories

The trends observed when applying QbD to the development of separation methods in pharmaceutical analysis are presented. Read more

16. Using Headspace Gas Chromatography for the Measurement of Water in Sugar and Sugar-Free Sweeteners and Products

This fast, automated method was shown to be accurate and precise for 16 liquid sweeteners, and is likely more accurate than Karl Fischer titration. Read more