The LCGC BLOG
Hydrophilic interaction chromatography (HILIC) can be a very useful tool in our analytical armory, however, there are some practical peculiarities to this chromatographic mode which need to be understood in order to ensure success. read more
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New! Zwitterionic, LC-MS columns for metabolomic applications are now available from The Nest Group™. Download the  brochure describing separations of nucleotides, phospholipids, amino acids, cell metabolites and artificial sweeteners, which includes literature references for an objective overview of capabilities for these silica and polymer based HILIC chemistries. Try iHILIC® Fusion columns or SPE cartridges today! The Nest Group, Inc. - "Not Just Columns ... Answers!" |
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LCGC TV
You can’t compromise on quality or cost in any analysis. Susan Olesik from The Ohio State University discusses how green chemistry does not involve any compromises. She also describes homogenous edge-plane carbon for use in reversed-phase HPLC. read more
LC TROUBLESHOOTING
Several variables can be used to change selectivity in a liquid chromatography (LC) separation. Here we compare the variables in an effort to prioritize which experiments will be most effective.
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Restek is bringing the speed and reliability of Raptor LC columns into the HILIC realm with the addition of the Raptor HILIC-Si column. The Raptor HILIC-Si column simplifies the switch to HILIC because it delivers rugged Raptor performance, provides SPP column speed for faster analyses than traditional FPP silica columns, retains polar compounds without ion-pairing reagents, and is fully reliable, efficient, and selective with LC-MS compatible mobile phases.  Learn more
NEWS
Researchers from Zhejiang University, China, have created a graphene-coated polymeric stationary phase and demonstrated its performance separating inorganic anions, organic acids, carbohydrates, and amino acids using anion exchange chromatography. read more
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Application Note
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The Agilent Intuvo 9000 gas chromatograph addresses issues associated with multiresidue pesticide analysis of food products.  Read more |
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FEATURED PRODUCTs
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The AZURA RID 2.1L is a sensitive and competitively priced differential refractometer, according to the company. It is suitable for detecting compounds with little or no UV activity such as alcohols, sugars, lipids, or polymers. This instrument is designed for use in analytical HPLC as well as for GPC and FPLC applications. The unit has advanced temperature control and ensures high sensitivity, fast baseline stabilization, and excellent reproducibility. Furthermore, the long-life LED, highly pressure resistant flow cell, improved safety features, and enhanced diagnostics functions offer easy handling and maintenance.
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Advanced Chromatography Technologies have introduced three exciting new selectivities for HILIC. These novel proprietary phases have been developed to enable chromatographers to achieve reproducible, successful HILIC separations.
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The alternative range of kits for HPLC pumps & autosamplers. Matching the performance of OEM equivalents, they cover Agilent & Waters instrumentation for next day delivery.
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PharmaFluidics’ game-changing chip-based chromatography column for nano-LC, lets you detect more molecules in tiny, complex biological samples. High resolution separations are obtained, perfect for lipidomic, metabolomic and peptide profiling.
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'Plug and play' generator to supply nitrogen for all LC/MS applications. Compact, low noise and long compressor life for continuous operation. Flow rates up to 30 lpm at 8 bar.
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The µDAWN™ is the world's first multi-angle light scattering (MALS) detector that can be coupled to any UHPLC system in order to determine absolute molecular weights and sizes of polymers, peptides, and proteins.
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