The LCGC BLOG

Going Back in Time – Ternary and Quaternary Gradients

Until recently, I hadn't heard of ternary or quaternary gradients being used for many years. They have gained a reputation for being somewhat difficult to reproduce—less robust, if you like. read more

DCM-Free, Semi Automated, Sample Cleanup for POPs Analysis

To meet demands for a lower cost method for the sample cleanup of dioxins and PCBs that requires little financial investment and eliminates hardware, features of the PowerPrep^® system to deliver consistent, fast, reliable results with short turnaround times and low background using FMS pre-packaged columns have been deployed. The SemiPrep™ with a simple semi-automated approach uses a minimum amount of solvent and zero DCM producing excellent results. Read more

LCGC TV

Retention Modeling in HILIC: What Works? and Predicting Retention in HILIC

HILIC retention is a highly complex mechanism. Gert Desmet from the Vrije Universiteit Brussel discusses his recent research into predicting retention in HILIC. He also reviews whether isocratic and gradient retention predictions could be made based on a limited number of runs. read more

FEATURE

Comparing the Separation Speed of Contemporary LC, SFC, and GC

Some 50 years after Giddings's iconic comparison of the separation speed of GC and LC, this article revisits this comparison using kinetic plots of the current state-of-the-art systems in LC, SFC, and GC. It is found that, despite the major progress LC has made in the past decade (sub-2-µm particles, pressures up to 1500 bar, core–shell particles), a fully optimized UHPLC separation is still at least one order of magnitude slower than capillary GC. The speed limits of packed bed SFC are situated in between. read more