LCGC Europe Application Note Alert Having trouble viewing this email? Click here Automated Method Development in Accordance with QbD Principles This article discusses the combination of Chromsword® Automated Method Development software with the power of a VWR Hitachi Chromaster HPLC or UHPLC system. Practical Ultra High Performance Liquid Chromatography (UHPLC) Increasing efficiency and resolution plus savings in solvent cost and analysis time have driven UHPLC uptake. This discussion outlines how technologies have evolved. Computer-Aided Method Development with Unique Column Selectivities This article introduces a streamlined approach to reversed-phase method development that combines column screening with advanced computer simulation software. Gradient Method Translation Using the ACE LC Translator Translating gradient LC methods from one column dimension to another is common, but maintaining the separation selectivity and peak resolution can be challenging. UHPLC Analysis of St John’s Wort Using Column Coupling Quantitative and qualitative analysis of natural products is important but is challenging. Chromatographic techniques that deliver high resolving power are needed. UHPLC Analysis of DNPH Derivatized Carbonyl Compounds This note shows the separation of 14 carbonyl compounds in a commercially available CARB Method 1004 test mix using sub-2-µm ACE Excel UHPLC particles in under 6 min. Vanillins—Fast Analysis A method for rapid separation of vanillin and seven related compounds under fast UHPLC conditions, screening vanilla extract authenticity using 1.7 µm particles. Gradient Method Transfer—Aspirin and Related Substances The importance of correcting for change in dwell volume between different systems when transferring a method is examined using the ACE translator tool.