LCGC Blog
I think it's fair to say that there has been a paradigm change in the way we approach mobile-phase design for HPLC in recent years.
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The molecular complexity of monoclonal antibodies (mAbs) makes accurate characterization challenging. This ebook highlights versatile and automated mass spectrometry-based approaches for intact protein analysis, peptide mapping, and glycan analysis that deliver quick and reliable answers.
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Features
What types of mixers are needed with different types of LC pumps? Are different mixers needed for different applications?
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IVT's 6th Lab Week conference series draws over 100 laboratory professionals and provides the value of three conferences in one, giving your team the ability to select sessions that best meet your business objectives. Hear from FDA and choose from over 25 workshops, think tanks, interactive presentations, case studies and educational courses surrounding analytical procedures and methods validation, stability testing and lab data integrity.
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News
Researchers from the University of Verona, in Verona, Italy, have investigated the diagnosis of diabetes mellitus in endurance athletes using HPLC.
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Get expert insights in the white paper "External reference standards or relative response factors: Considerations for quantitation of impurities in pharmaceuticals" from Mikromol.
This white paper is addressed to interested parties from pharmaceutical method development, validation and QC, and covers topics including:
- What are relative response factors?
- When and how do you set them up?
- What are the limitations of relative response factors?
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This instalment of ‘Perspectives in Modern HPLC’ provides an overview of modern trends and best practices in mobile phase selection in reversed-phase chromatography. The focuses are on selection criteria and rationales for enhancing analytical performance and ease of preparation.
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Application Note
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This application note shows the development of a multiresidue online SPE LC–MS/MS method coupled to direct injection for the analysis of 26 pesticide metabolites.  / Download Application Note / |
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FEATURED PRODUCTs
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The Eclipse® DualTec™ system offers both Hollow-Fiber Flow-FFF (HF5) andAsymmetric Flow-FFF (AF4) techniques. Both may be integrated into one instrument and coupled seamlessly to the most advanced light scattering detector for determination of molar mass and nanoparticle size, Wyatt's DAWN® HELEOS® II.  / Learn more / |
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PharmaFluidics' game-changing chip-based chromatography column for nano-LC, lets you detect more molecules in tiny, complex biological samples: excellent separation, unprecedented robustness and reproducibility, perfect for lipidomic, metabolomic and peptide profiling.
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Postnova introduces the powerful Triple Detection for Thermal Field-Flow Fractionation (FFF) and GPC/SEC. Triple Detection is the combination of Multi-Angle Light Scattering (MALS), Viscosity Detection, Refractive Index Detection and UV Detection. In a single separation experiment Triple Detection provides molar mass distribution, molecular size distribution and molecular structure (branching, composition) of polymers, biopolymers, polysaccharides, proteins and antibodies.
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HILICON HILIC columns, iHILIC®-Fusion, iHILIC®-Fusion(+), and iHILIC®-Fusion(P), are versatile for the LC–MS analysis of polar compounds in "Omics" researches, pharmaceutical discovery, and clinical diagnostics. In the application note, 14 amino acids can be directly separated by an iHILIC®-Fusion(+) column with a HILIC-MS setup. This method offers the possibilities to quantify polar amino acids in many application areas such as medicine, biology, or nutritional science.  / Learn more / |
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The GERSTEL MultiPurpose Sampler (MPS) with Filtration/DNPH Option performs clean-up of 240 samples using syringe filters, or elution of 72 DNPH cartridges combined with introduction to LC/MS - or GC/MS.
For more information about Filtration / DNPH elution and other GERSTEL solutions, please contact sales@gerstelus.com or visit  www.gerstel.com |
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The Bal Seal® combines advanced polymers with a wear-reducing spring energizer to dramatically reduce contamination, improve sample accuracy, boost throughput, and prolong service life. It helps pump designers meet demanding PM requirements of >1M cycles/60L.  / Learn more / |
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