FEATURES
In this article, two case studies regarding the characterization of protein-DNA complexes are presented, using two complementary multi-angle light scattering (MALS) techniques: SEC–MALS—to determine absolute molar mass of each component, with size-exclusion chromatography, and CG–MALS—to quantify stoichiometry and affinity of the interaction, label-free and in solution, with composition gradients. Read more
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Introducing the next generation DAWN multi-angle static light scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. DAWN offers high sensitivity, a wide range of molecular weight, size, and concentration, and a large selection of configurations and optional modules for enhanced capabilities.
 https://www.wyatt.com/dawn
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Challenges that can be encountered when using isocratic high performance liquid chromatography (HPLC) can be overcome by using gradient HPLC. Read more
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Dynamic light scattering (DLS), widely recognized for its ability to detect aggregation in purified protein solutions, is actually far more versatile and may be applied to the evaluation of stability and viscosity of therapeutic proteins and other biopharmaceuticals. In a fully automated, microwell plate reader format, DLS constitutes a high-throughput biophysical screening technique for early-stage assessment of candidate developability and optimal formulation conditions. This article explains more. Read more
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The microDAWN can be coupled to any UHPLC-SEC system to determine absolute molecular weights and sizes of polymers, peptides, or proteins directly, without resorting to column calibration or reference standards, and produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations.
 https://www.wyatt.com/microdawn
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The challenges we face in troubleshooting problems with liquid chromatography (LC) separations are highly diverse. In this article we take a closer look at topics that have garnered more attention recently. Read more
Comprehensive two-dimensional liquid chromatography (LC×LC) is a powerful technique for separating highly complex samples. However, the proliferation of this technique is hindered by a range of challenges, including the possible impact of the additional separation on the detection sensitivity, concerns that mobile phase incompatibility problems will limit the applicability, and the complexity of the system and associated method development costs. This article addresses these issues and describes how modern modulators and software tools are overcoming the barriers associated with this technique. Read more
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