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Simultaneous Quantification of Acetylcholine, Histamine, and Their Metabolites in Human Cerebrospinal Fluid (CSF)
Mary E. Lame, Erin E. Chambers, and Kenneth J. Fountain, Waters Corporation
The method described herein demonstrates the simultaneous quantification of ACh, HA, and their metabolites in human CSF. This application uses a single-step sample preparation, dilution of a 20–µL sample, followed by HILIC UPLC–MS–MS analysis. Using a sub-2–µm particle size, the high efficiency CORTECS UPLC HILIC column provided resolution from endogenous matrices, increased analyte senstivity, and a short analysis time of 2.5 min, thereby resulting in a fast, sensitive, selective, and accurate method that meets the demands of high-throughput bioanalytical drug discovery. Read More |
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Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma
Erin E. Chambers and Kenneth J. Fountain, Waters Corporation
Using analytical scale chromatography and simple 96-well SPE sample preparation, this method reaches detection limits for intact insulins, comparable to those previously achieved using immuno-precipitation and nano-scale chromatography. Read More |
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Analysis of Synthetic Cannabinoids from Whole Blood
Waters Corporation
A panel of 22 synthetic drugs and metabolites were extracted fro whole blood and analyzed by UPLC–MS–MS. The use of Ostro sample preparation plates resulted in excellent recoveries for all analytes with minimal matrix effects. Separation using the CORTECS UPLC column enabled the analysis of all compounds in a short analysis time with baseline resolution of a critical isobaric pair. Read More |
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Analysis of Synthetic Cannabinoids from Urine
Jonathan P. Danaceau, Erin E. Chambers, and Kenneth J. Fountain, Waters Corporation
A panel of 22 synthetic cannabinoid drugs and metabolites were extracted from urine and analyzed by UPLC–MS–MS. The use of Oasis HLB µElution plates enabled the simultaneous extraction of acidic, basic, and neutral compounds, ensuring that a wide variety of compounds and metabolites could be analyzed. Separation using the CORTECS UPLC C18 column enabled the analysis of all compounds in a short analysis time with baseline resolution of critical isobaric pairs. Separation efficiency and peak widths were superior to fully porous columns of matching dimensions and particle size. This method enables the rapid and reliable extraction and analysis of this critical class of compounds for forensic toxicology. The excellent performance seen on this variety of compounds and the universal nature of the extraction method should allow its use on other synthetic cannabinoids and metabolites, an important feature given the rapid development of new, related compounds. Read More |
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