Featured Application Note:
Quantitation of Metabolites in Plasma Samples by UV-MS Correction Using a Dual-cell - Linear Ion Trap Mass Spectrometer
By Tim Stratton, Yingying Huang, August Specht, Julie Horner, Thermo Fisher Scientific
The quantitative assessment of metabolites in samples from development in vivo studies has become an area of increasing study with the release of guidances from regulatory bodies (MIST and M3 R2) and their subsequent interpretation and implementation. The goal of this application is to quantify the desmethyl metabolites of dextromethorphan in plasma using a UV-MS correlation established through a standard curve generated in vitro. |
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Automated Solid Phase Extraction and Concentration for the Determination of Nitrosamines in Drinking Water
Phillip Bassignani, FMS, Inc
Nitrosamines are a group of chemical compounds regulated due to their carcinogenic characteristics. Nitrosamines have many sources including widespread usage in the manufacturing of rubber products, as well as cosmetics. They have also been demonstrated to form from reactions between food preservatives and amines. In drinking water, established limits are low (<1 ng/L), requiring detection by a Mass Spectrometer in the chemical ionization mode. |
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Solving Bottlenecks in Met-ID Data Analysis
Hesham Ghobarah, Alina Dindyal-Popescu and Hai Ying
This tech note summarizes how advanced new functions in MetabolitePilot Software from AB SCIEX help automate labor instensive aspecst of metabolism data interpretation. Newly integrated multiple sample correlation functionality for time course studies or inter-species comparison and integrated MS/MS fragment interpretation tools for performing structural elucidation are highlighted. |
A Variety of Agilent ZORBAX RRHD Phases Offers Selectivity Options for the Determination of Anthocyanins in Blueberries with UHPLC/MS
Anne E. Mack, Agilent Technologies
ZORBAX Rapid Resolution High Definition (RRHD) columns are stable to 1200 bar, and offer new selectivity options for UHPLC. The family now has more than twelve phases, including HILIC. This application note demonstrates and evaluates four different Agilent ZORBAX RRHD stationary phases are quickly evaluated: Eclipse Plus C18, Eclipse Plus Phenyl-Hexyl, StableBond SB-Aq, and StableBond SB-Phenyl. Glycosides and acylglycosides of five common anthocyanidins (cyanidin, delphinidin, peonidin, petunidin, and malvidin) are observed. |
Comprehensive Access to Proteomics Information
Stephanie Kaspar, Arnd Ingendoh, Christoph Gebhardt, Markus Meyer and Carsten Baessmann, Bruker Daltonik GmbH
Techniques and strategies are described that allow a deeper insight into proteomics questions. It is shown how technological innovations enable access to low-abundance, difficult-to-access proteins and simplify the detection of posttranslational modifications (PTMs). |
Fast Determination of Vanillin and its Synthesis Precursors
Xu Qun, Li Lang, and Jeffrey Rohrer, Dionex Products, now part of Thermo Fisher Scientific
This note describes a fast HPLC method to determine vanillin and its synthetic precursors for process monitoring and quality control of the two-step synthetic process. The lab also made the separation compatible with MS and used the MSQ to determine the identities of some of the synthesis byproducts. |
New generation monolithic silica columns for fast, high resolution drug separations without high pressures
Karin Cabrera and Egidijus Machtejevas, Merck Millipore
Using a novel new-design monolithic silica column (Chromolith® HighResolution RP-18e), fast, high resolution separations were achieved for various drug mixtures without the high pressures characteristic of modern particulate technology. |
Using pH to Control the Selectivity of Acidic and Basic Compounds on Gemini-NX
Lawrence Loo, Phil Koerner, Terrell Matthews, Jason Lam, Phenomenex
HPLC columns stable at pH extremes, like Gemini-NX, enable powerful selectivity manipulation and method flexibility to separate basic and acidic compounds when working in reversed phase mode. |
A Quality by Design (QbD) Based Method Development for the Determination of Impurities in a Peroxide Degraded Sample of Ziprasidone
Mia Summers and Kenneth J. Fountain, Waters Corporation
Method development can be a time-consuming process that can be repeated many times throughout a drug development pipeline. A systematic screening that evaluates a number of stationary phases, pH ranges and organic modifiers provides a thorough approach to method development. Incorporating Quality by Design (QbD) principles to this approach allows the use of a statistical design of experiments (DoE) to build-in robustness and define a 'design space', a region in which changes to method parameters will not significantly affect the results. |
Green Polymer Chemistry: Efficient Synthesis of Disulfide Polymers and Networks
Wyatt Technology
The objective of this experiment was to develop a greener synthesis of disulfide polymers which are reducible under specific biological conditions. |
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Quantification of Ethinyl Estradiol in Plasma at 1 pg/mL Using UPLC and Xevo TQ-S
Sunil Kumar, B. Tirupateswara Rao, P.M.N. Rajesh, and Gopal Vaidyanathan, Waters Corporation
In this study, an optimized combination of Waters technologies, including sample preparation chemistry, UPLC chromatography, and the Xevo TQ-S tandem quadrupole MS, enables determination of Ethinyl estradiol at its LLOQ of 1 pg/mL. |
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Analysis of polycyclic aromatic hydrocarbons from vehicle exhaust using TD–GC/ TOF MS
Paul Morris, David Barden, Markes International Ltd.
This Application Note describes how Markes International's UNITY 2 thermal desorber, in conjunction with high definition GC/TOF MS, can be used to detect polycyclic aromatic hydrocarbons (PAHs) in vehicle exhaust in a real-world situation. A key benefit of using direct thermal desorption is that it is much quicker and easier than traditional solvent extraction protocols and the combination of thermal desorption with high definition GC/TOF MS offers enhanced sensitivity and analytical performance for the application. |
The Analysis of 16 EPA PAH's
Mark Wilkinson and James Heseltine, Parker Hannifin Manufacturing Limited
To optimize and produce a robust and repeatable method for the analysis of 16 EPA PAH's by GC/MS using generated hydrogen as a carrier gas, over helium. |
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Excipient analysis by GPC/SEC and other LC techniques
Greg Saunders and Ben MacCreath, Agilent Technologies
In the past, excipients were thought of as cheap and inert substances whose sole purpose was to carry active ingredients. However, it is now recognized that they can influence the rate and extent of uptake of actives, and there is a move away from synthetic excipients towards 'natural' compounds that are potentially less toxic, more easily accessible, cheaper, and more acceptable to consumers in this age of health scares associated with synthetic products. This application booklet includes a wide range of applications that illustrate the performance of Agilent solutions for excipient analysis. |
Analysis of food additives by GPC/SEC
Greg Saunders and Ben MacCreath, Agilent Technologies
It is very important for food manufacturers that their products are consistent in taste, appearance and quality, and all these parameters can be affected by additives. Because many food additives are polymeric in structure they are best analyzed by gel permeation chromatography or size exclusion chromatography, which provide a thorough understanding of the characteristics of polymer additives. This compendium describes some of the uses of Agilent columns and systems for the gel permeation chromatography of common food additives. |
Analysis of biodegradable polymers by GPC/SEC
Greg Saunders and Ben MacCreath, Agilent Technologies
In polymer science and engineering, the design of polymers susceptible to biodegradation is of increasing importance – polymers that degrade naturally in the body to harmless products may be used in biological devices and in drug delivery, and polymers that break down in the environment are significantly ‘greener’ than traditional plastics. The rate of degradation of the polymer in the natural environment is related to the length of the polymer chains in the material. GPC is key to studying biodegradable materials by giving an insight into the rate at which a material might degrade. This application compendium shows examples of GPC applications involving different biodegradable polymers, derived from synthetic and natural sources. |
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Determination of Tetrafluoroborate, Perchlorate, and Hexafluorophosphate in Lithium Ion Battery Production
Duangchai Phesatcha, Dionex Products, now part of Thermo Fisher Scientific
This note demonstrates anionic content in the electrolytic solution in lithium batteries can be determined accurately using a Reagent-Free IC system (RFIC), which allows the analyst to avoid the problems encountered in eluent preparation. The RFIC system also delivers excellent retention time reproducibility for easy and reproducible quantification in the analysis of lithium ion battery electrolyte solutions. |
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