Application Note Alert

February 2010 Subscribe

Links to technical insights from sponsored Featured Application Notes on Chromatography Online.
Contact Editor
- Janet Kelsey

Featured Application Note:
High-throughput Screening Method for Multi-Class Antibiotics in Milk Using LC-MS/MS
By Linda Stolker and Ruud Peters, RIKILT; Richard Zuiderent, Joe DiBussolo, and Cláudia P. B. Martins, Thermo Fisher Scientific
This note presents a quick, high-throughput, sensitive method for simultaneous screening of multi-class antibiotic residues in milk by LC-MS/MS. It employs automated sample preparation technology to simplify sample preparation, reduce risk of operator error, improve sample throughput, and gain selectivity.

LC/HPLC
A Rapid Test for Pesticide Screening and Quantitation
By Dr. Andre Schreiber, Technical Marketing Manager, AB SCIEX

A Rapid iMethodTM Test for Pesticide Screening and Quantification including screening methods for 494 pesticides plus quantitation methods for carbamate, phenyl urea, triazine, organophosphorus, acidic and Japanese Positive List pesticides.
 

Simplifying Peptide Bioanalysis
By Erin E. Chambers, Jessalynn Wheaton, and Diane M. Diehl, Waters Corporation

Reliable, robust, sensitive and accurate bioanalytical methods need to be developed for biopharmaceuticals as well as for small molecules. Working with peptides presents many additional challenges over those typically faced with small molecules.

 

Method Transfer of Loratadine from HPLC to UPLC using the ACQUITY UPLC H-Class System
By Peter Alden, Waters Corporation

The compendial HPLC method for analysis of Loratadine is transferred to the quaternary-based ACQUITY UPLC H-Class System and migrated to UPLC. We illustrate that this new system is ideal for laboratories seeking improvements in efficiency and cost-effectiveness.

 

Megadalton protein complex characterization by MALS
By David Veesler, Wyatt Technology

Recent technological advances in macromolecular crystallography led to focus on the study of more and more sophisticated biological systems, such as protein-protein complexes.


GPC
Analysis of Complex Bacterial Cell Division Proteins by Size Exclusion Chromatography (SEC)
By Umbreen Ahmed, Varian, Inc

Analysis of proteins by SEC is a powerful tool for investigating the structure and aggregation of proteins. By separating proteins on the basis of size in solution, SEC can be used to identify and isolate proteins from such complex systems. This application note demonstrates the analysis of FtsZ and ZipA, two essential cell division proteins involved in septum formation in Escherichia coli (E. coli).


Hyphenated
Predicting drug/xenobiotic metabolism using on-line Electrochemistry/MS
By Hendrik-Jan Brouwer, Agnieszka Kraj and Martin Eysberg, Antec (USA)

With the introduction of the ROXY™ electrochemical system oxidative metabolism, as usually occurring in the liver cells by the Cytochrome P450 enzymes, can be simulated successfully within s and on-line detected by MS.


SFC
Introducing Mass-Directed Preparative SFC with Open-Bed Fraction Collection
By TharSFC, a Waters company

This application note describes the key innovation of SFC-MS Prep 100: gas-liquid separator. The software continuity from the analytical to the preparative platform and the orthogonality between SFC and RPLC are also commented.


Miscellaneous
Flash Chromatography: Alumina for Normal Phase Separations
By Stephen Ball, Varian, Inc

Flash, a rapidly growing technique in the field of chromatography, is essentially a simplified, rapid form of preparative LC. It offers savings both in time and cost of materials when compared to traditional prep chromatography. This application note demonstrates the differences in chromatography obtained from silica and alumina flash cartridges of different types, using a 2-component mixture of fat-soluble vitamins.

 
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+44 1244393110 | Email




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