Application Note Alert
March 2010 Subscribe
Links to technical insights from sponsored Featured Application Notes on Chromatography Online.
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- Janet Kelsey
Featured Application Note:
High Resolution and Mass Accuracy in Food and Feed Analysis
By Markus Kellmann, Andreas Wieghaus, Helmut Muenster, Lester Taylor, Dipankar Ghosh, Thermo Fisher Scientific
In complex sample matrices such as food, feed, hair, and honey, limited resolution leads to inaccurate mass measurements caused by unresolved background matrix interferences. This application note shows a full scan screening approach using a novel single stage Orbitrap™ mass spectrometer coupled to U-HPLC, capable of providing high mass accuracy at resolutions of up to 100,000.
LC/HPLC
Amioglycoside Antibiotics Analysis
By Hendrik-Jan Brouwer, Nico Reinhoud, Lusi van Heerwaarden, Antec

The ALEXYS® Aminoglycosides analyzer from Antec provides a sensitive and reliable solution for the analysis of impurities in Neomycin and Framycetin bulk drugs. The system is ideally suited for the analysis of these antibiotics in pharmaceutical formulations and bulk products including identification and quantification of their by-products and impurities.
 

Fast Screening for Vitamins and Dyes in Health Beverages with the ACQUITY UPLC H-Class System
By Mark Benvenuti, Waters Corporation

The ACQUITY UPLC H-Class System delivers a rapid and robust method for the analysis of 11 water-soluble vitamins and 3 food dyes in less than 8 minutes using a straightforward workflow.
 

Bottle-brush polymers
By Yan Xia and Robert H Grubbs, Wyatt Technology

Bottle-brush polymers are a type of high molecular weight (MW) graft polymers with very dense and regularly-spaced side chains.

 

A Rapid iMethodTM Test for the Screening of Antibiotics in Kidney Juice
By Dr. Andre Schreiber, Technical Marketing Manager, AB SCIEX

A Rapid iMethodTM Test for the screening and quantification of seven classes of antibiotics in kidney juice including and MRM catalogue with up to three transitions per compound that can be used to create customized methods.
GPC
Analysis of Polythiophenes via Conventional GPC
By Ian Willoughby, Varian, Inc.

Polythiophene is a class of polymer that contains a sulfur heterocycle in the polymer backbone. Polythiophenes are of paticular interest as a result of the their ability to conduct when electrons are doped into or from the conjugated backbone pi-orbitals. Analysis of polythiophene materials can easily be achieved by gel permeation chromatography (GPC) with Varian’s high efficiency PLgel 5 µm MIXED-D (300 x 7.5 mm) columns in conjunction with the PL-GPC 220 instrument equipped with RI detection.

Sample Prep
Determination of vitamin D2 and D3 in Human Plasma by on-line SPE.
By Martin Sibum, Spark Holland b.v.

A fully automated method for determining both 25-mono-hydroxy- Vitamin D2 and Vitamin D3 with no manual pretreatment step(s) using the Spark Symbiosis Pico™ on-line SPE and LC system has been created.
SFC
Using Mass-Directed Preparative SFC and RPLC for Library Compound Purification
By Andreas Mich, Burkhard Matthes, Rui Chen and Stefan Buehler, TharSFC, a Waters Company

The combination of two orthogonal techniques, mass-directed SFC and RPLC, for library compound purification resulted in an increase in success rate by 30% compared to using each technique alone.

Miscellaneous
Flash Purification of non-UV-active Compounds by Evaporative Light Scattering Detection
By Linda Lloyd, Stephen Ball and Keeley Mapp, Varian, Inc.

This note demonstrates the benefits of using a Varian 385-LC ELS detector alongside a 971-FP flash instrument for the analysis of a sample containing a mixture of UV and non-UV-active compounds. The major advantage of dual detection is the ability to prevent loss of sample components that remain undetected by UV, and are therefore not isolated by the fraction collector but are lost to waste.
 

Xevo TQ MS with APPI: The ionization of compounds with diverse structures using vitamins as a model
By Eleanor Riches, Waters Corporation

In this application note, we demonstrate that APPI can be used to analyze both compounds that would typically be ionized using ESI, and those that would typically be ionized using APCI.
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