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October 2015 Clinical/Forensic Food and Beverage Research and Development Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using Chiral HPLC and MS Detection The use of illicit drugs, amphetamine and methamphetamine, continues to be a high public health concern. Sample preparation using Supel™-Select SCX SPE allows for the removal of many interferences from urine. Rapid, baseline resolution of the enantiomers is achieved using chiral HPLC column Astec® CHIROBIOTIC® V2 and LC–MS detection. Download the app note Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC–MS of Serum or Plasma Samples Reducing matrix effect is an important consideration when developing an LC–MS method for serum or plasma samples. This brief report describes two distinct sample prep techniques to reduce matrix effect: targeted phospholipid depletion using HybridSPE®-Phospholipid and analyte enrichment using biocompatible SPME. Both methods provide effective means to reduce matrix interference that can rob the method of sensitivity, reproducibility, precision, and accuracy. Download the app note A Comparison of Two Solid Phase Extraction Methods for the Analysis of Polyaromatic Hydrocarbons in Butter This comparison shows that the Supelclean™ EZ-POP NP method produced better recoveries and lower background than direct SPE using a large silica cartridge for the analysis of PAHs from butter. When using EZ-POP NP, the final extract is clean enough for GC–MS analysis using the SLB®-35ms capillary column and single quadrupole instrument. Download the app note Extraction and Quantitation of Bisphenol A (BPA) from Broth-Based Soup Matrices Using Molecularly Imprinted Polymer Solid Phase Extraction This application illustrates the use of the SupelMIP® SPE-Bisphenol A for the extraction of BPA from beef and chicken broth matrices prior to chromatographic analysis. In addition to excellent analyte recovery and reproducibility, the MIP SPE extraction and cleanup method provided sufficient matrix interference removal, facilitating quantitation. In addition, this method allows for detection and quantification of BPA levels that were as low as 60 ng/g, which is lower than current specified EU regulations. Download the app note Quantifying Picogram Concentrations of Aflatoxin M1 in Liquid Milk Using SPE Cleanup and LC–MS Analysis Aflatoxins are produced by fungi and these species can infect important cereal grains, including maize, as well as ground (peanuts) and tree nuts. The focus of this study is the metabolite of AFB1, aflatoxin M1 (AFM1) that can be present in the milk of lactating dairy animals that have consumed aflatoxin contaminated feeds. A fast method for cleanup and analysis has been developed using Supel™ Tox AflaZea SPE and LC–MS. Download the app note HPLC Analysis of Water-Soluble Vitamins Using Titan™ C18 Column Water soluble vitamins are important compounds and are essential for normal metabolism. They are naturally found in many foods and are often added to processed food products. This analytical method used the Titan™ C18 column and UV detection resulting in excellent resolution of nine water soluble vitamins in 2.5 min. Download the app note Extraction of Permethrin Pesticides from Spinach Using QuEChERS Methodology with Automated Shaking QuEChERS was introduced in 2003 for simplified sample preparation in the analysis of pesticides in fruits and vegetables. In this article, permethrin was extracted from spinach using the Supel™ QuE 50 mL centrifuge tubes and following the QuEChERS procedure. Analysis was performed on capillary GC column SLB®-5ms with MS detection. Download the app note Scalable Specialty Carbon Adsorbents for Purification and Recovery Applications Specialty carbon adsorbents are used for purification and recovery applications in petrochemical, pharmaceutical, food and beverage, environmental, and other industries. Supelco® manufactures over 30 specialty carbon adsorbents, 16 of which are scalable. Carbon adsorbent sampler kits are available to help with adsorbent selection and we also offer custom capabilities. Download the app note Low Adsorption Autosampler Vials Supelco®’s Low Adsorption (LA) vials are manufactured using a process that decreases the number of hydroxyl groups on the vials glass surface, significantly reducing surface activity while improving analytical quantitation and minimizing pH shifts in the sample. For a successful analysis, vials must have lack of interference with the sample, proper fit, good seal, and correct septum. Download the app note Supelco SLB®-IL60 Ionic Liquid GC Columns: Improved Resolution The SLB®-IL60 gas chromatography (GC) column is based on an ionic liquid stationary phase platform and displays desirable features that existing non-ionic liquid columns do not. The SLB®-IL60 column is similar enough to PEG columns to make it useful for many of the same applications, but different enough to impart unique selectivity that can be leveraged to change elution patterns and improve resolution as shown in this article with industrial solvents. Download the app note   Go to Application Note Library Subscribe to the Application Note Alert LCE Print Edition LCE Digital Edition