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September 2015 Peptide Analysis Intact Proteins Analysis Glycans Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC System This application note proves the suitability of the Thermo Scientific™ Vanquish™ Flex UHPLC system for efficient and reliable peptide mapping experiments. The outstanding precision of retention times and peak areas on the biocompatible Vanquish Flex UHPLC system enables a routine peptide mapping analysis of biotherapeutics even without the use of mass spectrometry, based merely on UV data. Download the app note Providing the Highest Retention Time and Peak Area Reproducibility for Maximal Confidence in Peptide Mapping Experiments In this study, highly reproducible separation of the peptides of a monoclonal antibody was obtained with a 30-minute gradient and a total analysis time of 45 minutes, including column wash with high organic eluent, and re-equilibration at initial conditions. In average, the area relative standard deviation was 0.4%. Download the app note Reversed-Phase Separation of Intact Therapeutic Antibodies Using the Vanquish Flex UHPLC System This application note provides examples of reversed-phase chromatography of intact monoclonal antibodies with the Thermo Scientific™ Vanquish™ Flex UHPLC System and Thermo Scientific™ MAbPac™ RP column. High efficiency separation of antibody variants is easily achieved with a 10 minute long generic gradient. Thanks to the high efficiency and speed of separation of intact proteins, the method is an excellent tool for stability studies. Download the app note Development of Ultra-fast pH-Gradient Ion Exchange Chromatography for the Separation of Monoclonal Antibody Charge Variants Ultra-fast charged variant separations can be achieved as a result of several advances in chromatography, including high pressure–rated small-particle-size ion exchange columns, commercial buffer formulations used to form a linear gradient, and the use of a UHPLC system with extremely low delay volumes, high precision gradient formation, and a totally inert flow path. Download the app note Fast and Easy Optimization of Detection Wavelengths with Vanquish Fluorescence Detectors The goal of this technical note is to explain why the optimal fluorescence wavelength pair can vary between different fluorescence detector models and to demonstrate how users can easily verify and optimize fluorescence detection wavelengths with the Thermo Scientific™ Vanquish™ Fluorescence Detector. A 6-fold improvement of detection sensitivity is demonstrated for fluorescently labeled glycans by fine-tuning the excitation and emission wavelengths. Download the app note UHPLC Analysis of 2-Aminobenzamide-Labeled Glycans with the Vanquish Flex System This application note demonstrates the successful separation and detection of 2-aminobenzamide (2-AB)-labeled glycan libraries on a Thermo Scientific™ Vanquish™ Flex UHPLC system equipped with a Vanquish Fluorescence Detector F. Retention time precision for these glycan libraries as well as for a 2-AB-labeled single glycan standard are reported. Area precision and linearity are evaluated for three labeled glycan standards. Further, recommendations for the selection of the right detector flow cell are given. Download the app note   Go to Application Note Library Subscribe to the Application Note Alert Subscribe to the LCGC Europe print edition Subscribe to the LCGC Europe digital edition