Featured Application Note:
Analysis of Bisphenols in Canned Food by LC-MS/MS
By Héctor Gallart-Ayala, Encarnación Moyano, M.T. Galceran, Cláudia P.B. Martins
A fast and sensitive method for the simultaneous analysis of BADGEs and BFDGEs in canned food and beverages is described. |
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One Step Extraction, Cleanup and Concentration of PCBs in Soil
Phil Bassignani, FMS, Inc.
Due to PCB's toxicity and classification as a persistent organic pollutant, PCB production was banned by the United States Congress in 1979 and by the Stockholm Convention on Persistent Organic Pollutants in 2001.PCBs have low water solubility and are largely chemically inert, making them extremely resistant to oxidation. These properties enable PCBs to with stand environmental degradation, and build up in soil and river sediment. This note discusses automating the extraction,cleanup and concentration of soil samples in one step. |
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Rapid Metabolite Identification using MetabolitePilot™ Software
Carmai Seto, Tanya Gamble, Hesham Ghobarah, AB SCIEX
This technical note describes how MetabolitePilot™ software and the AB SCIEX TripleTOF™ 5600 System can make the process of identifying and confirming metabolites more efficient than ever before. |
Determination of Lactose in Lactose-Free Milk Products
Pranathi Perati, Dionex Corporation
This note demonstrates a rugged method for the determination of lactose in lactose-free milk products by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection. The same method can also measure lactulose which is produced at varying levels in milk depending on the method used to sterilize. |
A Simple Method for Analyzing Aggregates of EPO (Erythropoietin) Using a BioSep™-SEC-s2000 GFC Column (TN-1101)
Michael McGinley, Phenomenex
Gel filtration chromatography is the primary method used to analyze the amount of aggregate and dimer present in a therapeutic protein sample. A rugged yet simple method using a BioSep-SEC-s2000 column is presented for analyzing EPO samples to determine difference in the amounts of aggregate present in both fresh and longterm stored EPO samples. |
Absolute Molecular Weights of Heparin
James Knobloch and Dr. Patrick Shaklee, Wyatt Technology
The U.S. Food and Drug Administration (FDA) has scrutinized LMW-heparin products closely due to their inherent variability and polydispersity. Fortunately, the advent of multiangle laser light scattering combined with SEC means that authentic calibration standards derived from heparin are no longer necessary. |
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Highly Sensitive UV Analysis with the Agilent 1290 Infinity LC System for Fast and Reliable Cleaning Validation – Part 3
Edgar Naegele, Katja Kornetzky, Agilent Technologies
This Application Note studies detection of the contamination of an active pharmaceutical product (APP) with the remains of another APP. It demonstrates that high sensitivity detection of very low level amounts of contaminant with the Agilent 1290 Infinity LC DAD equipped with a high sensitivity 60 mm flow achieves about five times higher sensitivity than the standard cell. |
Analysis of Oxidized Insulin Chains using Reversed-phase Agilent ZORBAX RRHD 300SB-C18
Phu T Duong and Linda Lloyd, AB SCIEX
The use of a new Agilent ZORBAX RRHD 300SB-C18 1.8 µm column designed for UHPLC systems significantly reduced analysis time, critical for increasing the efficiency of QC for protein primary structure analysis. The separations also demonstrated how this technology achieved resolution of various insulin isoforms. By using StableBond technology it was possible to produce a 300Å pore-size media that was stable under acidic conditions, to provide the robust reproducible separations required for protein QC. |
Water- and Fat-Soluble Vitamins in Nutritional Supplements by HPLC with UV Detection
Luo Ji, Xu Qun, Li Lang, and Jeffrey Rohrer, Dionex Corporation
This note describes a fast and efficient HPLC method to determine the vitamin contents of five different nutritional supplement tablets. |
Straightforward HILIC-MS/MS Analysis of “Leather Milk”
Jeffrey Lei, Sanjay Poman, Gora Sharangi and Patrik Appelblad, Merck Millipore
HILIC-MS or HILIC-ESLD can potentially be used for qualitative analysis of "Leather Milk", but to positively identify and allow quantitative analysis, tandem mass spectrometric detection is necessary. In this application note, we have introduced a new rugged and reliable method using HILIC-MS/MS for analysis of milk samples using L-hydroxyproline as marker. |
Evaluation of New HPLC/UHPLC Technologies for the Separation of Preservatives in Cosmetics
Terrell Matthews, Zeshan Aqeel, & Jeff Layne, Phenomenex
This study evaluates the performance of five different HPLC columns used in a fast, selective screen to determine several common preservatives in cosmetics. The work demonstrates the higher performance of three core-shell silica products (Kinetex® XB-C18, HALO® C18, and Poroshell 120 SB-C18) compared to two traditional fully porous HPLC columns (Luna® C18(2) and XSelect™ CSH™ C18). |
Quantitative Analysis of Catechins in Tea by UHPLC/UV
Guifeng Jiang, Terry Zhang, Thermo Fisher Scientific
This analysis features robust UHPLC/UV methods for high-throughput screening and quantitation of catechins and other phenols in tea matrices. These methods can be adapted for analysis of other foodstuffs and for studies examining the effects of catechin intake on human health. |
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To advertise or have an application note inserted, contact:
Lee Noyes
+44 1244 629315 | Email
Lindsay Jones
+44 1244 629316 | Email
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