|
|
 |
|
 |
Transfer of a USP Method for Prednisolone From Normal Phase HPLC to SFC Using the Agilent 1260 Infinity Hybrid SFC–UHPLC System
Syed Salman Lateef, Agilent Technologies
Normal phase liquid chromatography (LC) methods often have long run times and involve environmentally toxic or costly solvents. Supercritical fluid chromatography (SFC) methods on the other hand are faster, inexpensive, and eco-friendly. SFC involves the use of low viscosity supercritical carbon dioxide that can be operated at flow rates up to 3x higher than LC without losing separation efficiency and thereby leading to faster analysis. In this work, a normal phase United States Pharmacopeia (USP) prednisolone assay method is transferred to a SFC method. The results show that the SFC method meets the system suitability criteria, demonstrates excellent robustness, is 4x faster, and is 17x lower in solvent expenses. |
Rapid Determination of Phthalates
Dr. Silvia Marten and Mareike Margraf, KNAUER
A fast method for the separation and determination of eight commonly used phthalates applying the KNAUER PLATINblue UHPLC-HPLC system is presented in this application note. Reduction of analysis time from about 35 to less than 5 min compared to a conventional HPLC method is achieved by using the BlueOrchid C18 stationary phase with a 1.8 µm particle size filled in a 2 mm i.d. column. |
|
|
|
 |
Low-Molecular-Weight Heparins
Lin Rao and John Bierne, Wyatt Technology
Size-exclusion chromatography (SEC) has been the most common way of measuring the molecular weight and molecular weight distributions of LMWHs by using the two most common detection technologies: ultraviolet (UV) coupled with refractive index (RI) detection. However, these detectors embody a relative method to determine molecular weights, requiring calibration standards. |
|
|
|
 |
Semivolatile Analysis with Specially Designed Agilent J&W DB-UI 8270D GC Column
Ken Lynam, Agilent Technologies
There is a wide range of volatile and semivolatile contaminants finding their way into water sources around the world. While finding analytes of interest is approached differently world-wide, it is clear that water quality needs to be monitored at a global basis. This app note demonstrates excellent peak shapes and chromatographic performance for challenging semivolatile analytes on the Agilent J&W DB-UI 8270D Ultra Inert GC column. |
Method 8260C by Purge and Trap GC–MS
Ruben Garnica and Dawn May, PerkinElmer, Inc.
U.S. EPA Method 8260C – Volatile organic compounds (VOCs) by gas chromatography–mass spectrometry (GC–MS) is one of the most common environmental applications for GC–MS. This method outlines the analysis of volatile organic compounds in a variety of solid waste matrices including various air sampling trapping media, ground and surface water, soils, and sediments among others. The study presented here demonstrates the PerkinElmer® Clarus® SQ 8 GC–MS with purge and trap sample introduction both meets and exceeds the performance criteria set out in method 8260C and describes the analytical results and instrumental methodology. |
|
|
|
 |
Automated Solid Phase Extraction (SPE) and Concentration of Organochlorine Pesticides in Water
Phillip Bassignani, FMS, Inc.
Organochlorine pesticides are man-made organic chemicals with a history of wide spread use throughout the world. Tending to be very persistent in the environment, they have found their way into sediments and drinking water supplies posing serious health risks. The automated solid phase extraction and concentration method described allows for rapid, reproducible extractions using minimal solvent volume that produces consistent results. |
Determination of Polycyclic Aromatic Hydrocarbons(PAHs) in Seafood Using GC/MS
Don Shelly and Craig A Perman, UCT
This method is used for the determination of polycyclic aromatic hydrocarbons(PAHs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers(PBDEs) in fish and seafood--oyster, shrimp, and mussel. Benzo[a]pyrene is theprimary analyte of interest. GC/MS instrumentation is used for analysis. |
Extraction of Immunosuppressants from Blood using ISOLUTE SLE+
Rhys Jones, Lee Williams, Gavin Jones, Biotage
The sample prep method in this application note offers an alternative approach to immunosuppressant analysis with LC-MS/MS giving more reliable and robust recoveries with no cross-reactivity and cost saving opportunities. Analyte recoveries range from 60-97% with RSDs all below 10%. |
|
|
|
|
|
|
To advertise or have an application note inserted, contact:
Lee Noyes
+44 1244 629315 | Email
Lindsay Jones
+44 1244 629316 | Email
|
| |
|
