September 2012 Subscribe Go to Application Notebook

Fluorescence of Cutting Oils Lilian Norris and Yvette Mattley, PhD, Ocean Optics Cutting oils used in machining processes can leak and contaminate the environment or harm workers. In this application, we used a QE65 Pro spectrometer to discriminate among five sample oils.   Quantitation of Protein Using a Micro-Volume UV Spectrophotometer Shimadzu Protein analysis is commonly carried out in life sciences laboratories and there is a need for a rapid and simple analysis tool for protein samples. This note demonstrates fast, quantitative measurement of the amount of protein in a sample using 0.2 mm and 0.7 mm pathlengths.   Handheld Raman Spectroscopy for Inspection of Raw Materials B&W Tek, Inc. In this application note, we discuss the advantages of handheld Raman spectroscopy for the identification of unknown compounds.   Raman, PL & AFM Imaging on Laser-Lithographically Written Si-Structures WITec GmbH This application note describes structures in crystalline Si created using laser lithography, which were then examined with the same instrument using confocal Raman imaging, confocal PL imaging, and AFM.   1064 nm Dispersive Raman Systems in Biofuel and Plant Research Huawen (Owen) Wu, Eric Bergles, and William Yang, BaySpec, Inc. Raman now works on highly fluorescent plant-based samples without sample preparation.  1064 nm dispersive Raman is a viable new option for users who are studying highly fluorescent samples such as plants and biofuels.   Protein Stability by Chemical Denaturation Sebastian Fiedler, Lindsay Cole, and Sandro Keller, Applied Photophysics and the Molecular Biophysics Group at the University of Kaiserslautern Circular dichroism (CD) is a sensitive probe of protein conformation and is often used with chemical denaturation to obtain ΔG° in titration experiments. Here we present an automated CD batch method that offers several advantages over such titrations.

Determination of Silicon in Diesel and Biodiesel by Microwave Plasma-Atomic Emission Spectrometry Renata S. Amais, George L. Donati, Daniela Schiavo, and Joaquim A. Nóbrega, Agilent Technologies This app note describes the analysis of Si in diesel and biodiesel samples using a new elemental analysis technique — microwave plasma-atomic emission spectrometry (MP-AES). Considering cost, performance, and multi-element capabilities, the Agilent 4100 MP-AES is a suitable and efficient alternative to flame AA for this application.   Measurement Analysis of Chinese Herbal Medicines by Microwave Plasma-Atomic Emission Spectrometry (MP-AES) Chunhua Wu, Yuhong Chen, Kun Ouyang, Zhixu Zhang, and Craig Taylor, Agilent Technologies A rapid, simple, and accurate method for the determination of key elements in Chinese herbal medicine samples using a new elemental analysis technique — microwave plasma-atomic emission spectrometry (MP-AES). MP-AES provides an ideal, low-cost solution for multi-element analysis of TCMs, with excellent long-term stability, reduced running costs, and improved lab safety.   ICP Analysis of Additive Elements in Lubricating Oil Shimadzu Analysis of additives, consisting of various organic metal substances, is important to ensure quality management. ICP emission spectrometry is an effective method for this analysis; this note presents data on the analysis of additive elements in commercially available engine oil, ATF oil, and gear oil.   Analysis of Elemental Content in Commercial Chocolate Bars Patricia Atkins, SPEX CertiPrep This study examined the elemental composition of several types of chocolate products from chocolate liquor to milk and dark chocolate bars.   RoHS Rapid Screening Rigaku Corporation The restriction on hazardous substances (RoHS) has been in effect for several years and regulates the maximum allowable levels of some heavy metals in consumer goods, particularly in plastics and polymers. Measurement of Cr, Hg, Pb, Br, and Cd in polyethylene (PE) by XRF rapid screening using the Rigaku NEX QC instrument with an empirical calibration method is discussed.