December 2011


December 2011	Subscribe	Go to Application Notebook

Quantitation and Identification of Pharmaceuticals and Personal Care Products (PPCP) in Water Samples
André Schreiber and Rolf Kern, AB SCIEX
Because PPCP encompasses such a wide range of pollutants, it can make method development to monitor and assess these compounds challenging. A method is presented for analyzing 80 EDC and PPCP compounds using LC–MS–MS. This method is a straight-forward approach for the analysis and identification of these compounds with excellent sensitivity and ruggedness.

Quantitation and Identification of the Pesticide Malathion in Fruit Samples using MRM³ Quantitation
André Schreiber, Tania Sasaki, and Tanya Gamble, AB SCIEX
LC–MS–MS methods were developed to quantify and identify the organophosphorus pesticide malathion in fruit samples using the new QTRAP^® 5500 LC–MS–MS System. Both traditional MRM and MRM³ methods were compared regarding selectivity, sensitivity, accuracy, and reproducibility. The results show that the higher selectivity of MRM³ eliminates background and matrix interference, resulting in better data quality.

Detection of Allergens in Bread and Pasta by Liquid Chromatography Tandem Mass Spectrometry
Stephen Lock, Cathy Lane, Phil Jackson, and Antonio Serna, AB SCIEX
With the prevalence of food allergies on the rise, there is a need amongst food producers and regulators for specific and sensitive methods to detect allergens at trace levels. A rapid, robust, sensitive, and specific LC–MS-MS assay has been developed for the simultaneous detection of four major food allergens peanut, milk, wheat, and egg, which significantly simplifies sample preparation.

Rapid Metabolite Identification: Easily Find, Identify and Confirm Metabolites in Accurate Mass Data
Carmai Seto, Tanya Gamble, and Hesham Ghobarah, AB SCIEX
The ability to find, identify, and confirm metabolites as quickly as possible is critical at multiple stages of drug discovery and development. Advances in hardware and software have made the process of identifying and confirming metabolites more efficient than ever before.

Solving Bottlenecks in Metabolite ID Data Analysis
Hesham Ghobarah, Alina Dindyal-Popescu, and Hai Ying, AB SCIEX
Data interpretation in metabolite identification studies is one of the most time consuming activities performed by the biotransformation scientist. How advanced new software functions can help automate those labor-intensive aspects of data interpretation, allowing the scientist to devote more time to decision-making.

MS/MS^ALL with SWATH Acquisition
Stephen Tate and Christie Hunter, AB SCIEX
In many samples, the complexity and dynamic range of compounds is very large posing challenges for analysis. MS/MS^ALL with SWATH Acquisition enables comprehensive acquisition and targeted quantification for the highest quality data.

Analysis of Intact and Reduced Therapeutic Monoclonal Antibodies Using the TripleTOF™ 5600 System
Eric Johansen, Jenny Albanese, and Christie Hunter, AB SCIEX
The TripleTOF™ 5600 System provides an excellent solution for the study of intact heterogeneous therapeutic antibodies. Molecular mass, degree of glycosylation, N-terminal blocking, pyroglutamatic acid conversion, and changes in the stoichiometry of protein degradation can be determined using the sophisticated mass reconstruction tools in BioAnalyst™ Software.

Protein Biomarker Research Pipeline for Developing Protein Biomarkers for Diabetic Kidney Disease
Scott Bringans, Proteomics International; Thomas Stoll, Kaye Winfield, Tammy Casey, and Richard Lipscombe, Proteomics International & Centre for Food and Genomic Medicine; Tim Davis, University of Western Australia; and Jenny Albanese, AB SCIEX
This note explains how an AB SCIEX customer developed a biomarker pipeline using TOF/TOF™ 4800 and 4000 QTRAP^® Systems for discovery and verification/validation of biomarkers. It describes finding protein biomarkers for early detection of diabetic nephropathy, using iTRAQ^® reagent technology for discovery and MRM quantitation for verification/validation.

Contact Editor - Meg Evans

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