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| Links to technical insights from sponsored Application Notes on Spectroscopy Online. |
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Analysis of Acetaminophen
Nicole Kreuziger Keppy, Gordon Bain, Ph.D., and Michael W. Allen, Ph.D., Thermo Fisher Scientific
UV-Vis is an essential QC tool useful for identity, purity, and quantitative analysis assays of raw materials, active ingredients, and final products in pharmaceutical manufacturing. Assays for pharmaceuticals can easily be performed using the Thermo Scientific Evolution Array UV-Visible spectrophotometer and VISIONcollect software. In this application note, the quantity and percent assay of acetaminophen is determined according to USP methods. |
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Addressing Complex Sample Matrices
Nicole Kreuziger Keppy, Thermo Fisher Scientific
One of the difficulties in analyzing samples with complex matrices such as vaccines, injectable pharmaceuticals, and nutritional supplements is that they often contain additives which exhibit a significant absorption in the same wavelength range as the analyte of interest. This problem can lead to inaccuracies in collected data and methods for these samples can be time consuming to perform. The Evolution 300 UV-Vis spectrophotometer equipped with two cell changers provides a simplified solution to this problem. |
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Analysis of Nitrate Nitrogen (NO3- ) in Water by the EPA Approved Brucine Method
Gordon Bain, Ph.D., Michael W. Allen, Ph.D., and Nicole Kreuziger Keppy, Thermo Fisher Scientific
The U.S. Environmental Protection Agency (EPA) requires public water supplies to keep nitrates in drinking water within safe levels. In this experiment, quantitative analysis of nitrate nitrogen (NO3- ) in water was successfully performed with the Evolution Array spectrophotometer and VISIONcollect software. Rapid acquisition of full-range spectra and excellent instrument sensitivity makes this analysis quick, accurate, and reproducible. |
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Determination of Free Glycerol in Biodiesel
Nicole Kreuziger Keppy, Gordon Bain, Ph.D., and Michael W. Allen, Ph.D., Thermo Fisher Scientific
Free glycerol, a byproduct of the manufacture of biodiesel, affects the quality of biodiesel, and its content is regulated by industry standards. The sensitive measurement of the amount of free glycerol in biodiesel is measured with a Evolution Array UV-Vis spectrophotometer for conformance to American (ASTM) and European (EN) standards. |
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Wine Color Analysis
Gordon Bain, Ph.D., Thermo Fisher Scientific
The ability to measure all wavelengths from 190–100 nm simultaneously makes the Evolution Array UV-Vis system ideal for performing quality control measurements for wine color. Color analysis software automates the process of calculating color properties according to international protocols and presents the results to the user for record keeping and reporting. |
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The Determination of HMF in Honey
Nicole Kreuziger Keppy and Michael W. Allen, Ph.D., Thermo Fisher Scientific
Hydroxymethylfurfural, or HMF, is an aldehyde that is often used as an indicator for the quality of honey. Although HMF is not thought to be a harmful substance, food standards in many countries regulate the levels of HMF in honey. The HMF content of honey is determined by the White Method quickly and easily with an Evolution Array UV-Visible spectrophotometer. |
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Determination of Film Thickness
Gordon Bain, Ph.D., Michael W. Allen, Ph.D., and Nicole Kreuziger Keppy, Thermo Fisher Scientific
The Evolution Array UV-Vis spectrophotometer, combined with a Specular Reflectance Accessory, provides fast, convenient, and accurate measurement of film thickness. It can be used to measure thin films on the order of a few microns thick for applications such as photoresist films on semiconductors, LCD monitors, nano-gel particles, optical lenses, and anodized coatings. |
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Measuring the UV Protection Factor of Fabrics
Michael W. Allen, Ph.D. and Gordon Bain, Ph.D., Thermo Fisher Scientific
Scientific methods of evaluating UPF factors of fabrics have been developed and specified by Australia/New Zealand (AZ/NZ) standards. Fabric UPF measurements are fast and easy with the Thermo Scientific UPF measurement system, custom designed for the textile and garment industry. |
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Enzymatic Colorimetric Methods for the Analysis of Human Serum
Nicole Kreuziger Keppy, Gordon Bain, Ph.D., and Michael W. Allen, Ph.D., Thermo Fisher Scientific
High cholesterol and blood sugar disorders are a health concern for many adults resulting in chronic health problems, such as heart disease and diabetes. In this application note, we examine two enzymatic colorimetric methods, one for total cholesterol and the other for glucose using a sample of human serum. Human serum is analyzed quickly and easily with the Evolution Array UV-Vis spectrophotometer, which delivers reliable, full-spectrum data in less than 1 s. |
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The Biuret Method for the Determination of Total Protein
Nicole Kreuziger Keppy and Michael W. Allen, Ph.D, Thermo Fisher Scientific
Automated quantitative analysis of protein is fast and efficient using the Evolution Array UV-Vis spectrophotometer. The software includes a preconfigured method for the Biuret assay, allowing customization to individual laboratory protocols. Integration of the sample measurement and data analysis into the software saves time and improves laboratory throughput by eliminating post-measurement data manipulation. |
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Analysis of Methylene Blue Reduction by Ascorbic Acid
Nicole Kreuziger Keppy and Michael W. Allen, Ph.D., Thermo Fisher Scientific
The mechanism and rate of chemical reactions can be determined using UV-Vis spectrophotometry. In this application note, the reaction of methylene blue with ascorbic acid is monitored with the Evolution Array UV-Vis spectrophotometer and the rate constant is determined using the VISIONcollect software. |
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