Event Overview:

Many protein-based biopharmaceuticals are glycosylated proteins. The glycans themselves directly affect the therapeutic efficacy and safety of the biopharmaceutical product. Analysis of the sites of glycan attachment, the glycans that are present, and the relative proportions of each alternative structure are required for regulatory reasons. The number and similarity of possible structures of these molecules place tremendous demands on the analytical techniques. Due to the biological significance of glycoproteins, rapid, efficient, sensitive, and reproducible analytical methods are needed for qualitive and quantitative glycan analysis. Recent advances in chromatographic technologies, fluorescence-based direction, and mass spectrometry approaches to the characterization of protein glycosylation have led to better resolution, sensitivity, and throughput for greater reliability in analysis of protein glycosylation in development and quality control.

 
Who Should Attend:

By focusing on liquid chromatography (LC) methodolgy as applied to glycan analysis, this webinar will be advantageous for:

• Biochemists who identify and characterize biotherapeutic proteins
• Discovery scientists analyzing post-translational modifcations of proteins
• Development scientists involved in process optimization of cultures
• Pharmaceutical chemists who develop formulations and test stability
• Quality control chemist monitoring biopharmaceuticals for quality and consistency
• Developers of follow-on biologic

Key Learning Objectives:

Key learning points for this webinar include:

• Role of glycan analysis in characterizing modern biotherapeutics
• Advantages available with the application of UHPLC columns and instrumentation designed for glycan separations
• The application of UHPLC glycan analysis in a biopharmaceutical characterization laboratory
• The use Q-TOF mass spectrometry for glycan characterization in combination with sophisticated informatics tools