Downstream scale-up purification of influenza virus using ReadyToProcess equipment

This application note describes a case study where live influenza virus was purified using single-use ReadyToProcess technology. The ReadyToProcess platform is a flexible manufacturing solution, which facilitates easy handling of virus solutions and helps improve productivity. As model system for a live, attenuated influenza virus vaccine, a A/H1N1/Solomon Island strain produced in Vero cells was used. Total process time for the downstream purification was 3 days and the overall scale-up process resulted in production of influenza virus with the same yield and purity as obtained in the laboratory scale.
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Preparation and High Resolution Analysis of a Fab

MAb fragments are getting increased attention in research use due to their small size and lower immunogenicity (relative to intact antibodies). In this work, Fab was produced by papain cleavage of a purified monoclonal antibody (MAb) followed by a purification on MabSelect Sure™ and Capto™ L media (Protein A and Protein L immobilized to high flow agarose, respectively). Fractions from the different steps were analyzed with a new size exclusion chromatography (SEC) medium, Superdex™ 200 Increase.
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