CaPure-HA

Hydroxyapatite Resin for Biomolecule Purification
  • Multipurpose chromatography media with functional groups at multiple active sites.
  • Ideal for aggregate removal for mAbs.
  • Robust enough to withstand the rigors of use in commercial scale manufacturing operations.

mAb aggregate removal with
CaPure-HA


Resin:
Column:
Mobile phase:

Gradient:
Flow rate:
Detection:
Sample:
Sample load: 		CaPure-HA
1.0 cm ID × 10 cm (7.85 mL)
A: 5 mmol/L sodium phosphate, pH 6.8
B: mobile phase A + 2.0 mol/L NaCl
0-100% mobile phase B (20 CV)
3.93 mL/min (300 cm/hr)
UV @ 280 nm
protein A purified mAb, 10.0 g/L
10.0 g/L-resin

mAb pre- and post-purification on
CaPure-HA


Column: 

Mobile phase:
Gradient:
Flow rate:
Detection:
Injection vol.:
Sample: 

TSKgel® G3000SWXL, 5 µm,
7.8 mm ID × 30 cm
0.2 mol/L phosphate buffer, pH 6.8
isocratic
1.0 mL/min
UV @ 280 nm
20 µL
mAb IgG1 pre- and post-purification


For more information and to order CaPure-HA, please visit our website.


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King of Prussia, PA 19406
Customer Service: 866-527-3587
Technical Service: 800-366-4875, option #3
www.tosohbioscience.com


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