Understanding Gradient HPLC
  The CHROMacademy Essential Guide to Understanding Gradient HPLC  
LIVE WEBCAST
Tues. 14th December 2010,
11:00 EST, 4:00 BST
 
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CHROMacademy announces the next in our highly popular series of FREE LIVE TUTORIAL events
hosted in conjunction with the CHROMmunity

Understanding Gradient HPLC

The Essential Guide from LCGC’s CHROMacademy presents the definitive practical guide for users of Gradient HPLC.  In this session, Mike Schwartz (Research Director, Synomics Pharmaceutical Services) and Tony Taylor (Crawford Scientific Technical Director), present a definitive guide to using, optimizing and troubleshooting Gradient HPLC, all supported by Interactive Multi-Media from The CHROMacademy.  The session will consider key topics such as the mechanisms of Gradient HPLC versus isocratic elution, developing robust gradient HPLC methods, the dangers of altering column flow or column dimensions without proper gradient scaling and the essentials of troubleshooting gradient issues.  A must see for everyone using or developing Gradient HPLC methods.



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KEY LEARNING OBJECTIVES
  • Understand the advantages and limitations of Gradient HPLC
  • Gain insight into the working principles of Gradient HPLC and be able to contrast the elution model with Isocratic HPLC
  • Be able to implement gradient HPLC development strategies and appreciate the difference between linear and non-linear gradients
  • Understand the requirements for robustness in Gradient HPLC, including the requirements for reproducible gradient formation, initial isocratic holds and proper column re-equilibration
  • Understand how to transfer gradients when changing the eluent flow rate and/or column dimensions without compromising the selectivity of the separation
  • Be able to troubleshoot Gradient Issues including irreproducible retention times, unstable separation selectivity / resolution, noisy or rising baseline and ghost peaks.
WHO SHOULD ATTEND
  • Current or potential users of Gradient HPLC and those wanting to improve their skills and understanding of this technique
PRESENTERS:
Tony Taylor   Tony Taylor
Technical Director
Crawford Scientific
Mike Schwartz   Mike Schwartz
Research Director
Synomics Pharmaceutical Services
Peter Houston k

Peter Houston
Editorial Director
LCGC Europe

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