LIVE WEBCAST: Wednesday, October 20, 2010 at 11:00 AM PT, 2:00 PM ET, 7:00 PM GMT
Effective Implementation of Mass Spectrometry in the Analysis of Biotherapeutics
For many years, Mass Spectrometryhas been regarded as a very difficult, highly specialized analytical technique that, while powerful, was not appropriate for implementation into front line Analytical Development or QC operations. But, those days seem to be a thing of the past as improvements in hardware, software, and instrumentation technology have led to the development of instruments and applications that can be, and are being, deployed in Development operations. In this presentation, a series of Applications of Mass Spectrometry to the analysis of Biotherapeutics will be reviewed and detailed. These applications include: Complete, Direct Sequence Analysis of Intact Proteins and Peptide Digests; Detection and Identification of Protein Modifications (Truncations and Splice Variants, Point Mutations, Signal Peptides, Fusion Proteins); Identification of Sites of Post Translational Modifications (Phosphorylation, Glycosylation); Analysis of Glycans (N- and O-Linked Sugars). With the ever increasing numbers of protein and peptide therapeutics moving through the Development process at many companies, the need for fast, robust, effective, and powerful methods for detailed analysis of biotherapeutics has become acute. Current methods for protein analysis are often very slow, labor intensive, and provide limited information. Mass Spectrometry is the definitive tool for protein analysis. This presentation will demonstrate how the incorporation of Mass Spectrometry in routine analyses can be done, and explain many of the benefits of adopting a Mass Spec approach to analyzing biotherapeutics.

LIVE WEBCAST: Tuesday, October 26, 2010 at 11:00 AM EST, 8:00 AM PST
Increase LC/MS Throughput and Improve ROI for Biological Samples
Researchers working with biological samples are focused on improving productivity while reducing costs. One way to dramatically impact productivity is to improve the efficiency and throughput of your LC-MS system without sacrificing data quality. This webinar will present new strategies with advanced multiplexing and automated online sample preparation. We will present a case study based on this strategy as a tool to analyze 45 drugs of abuse in performance horses.

LIVE WEBCAST: Wednesday, November 3, 2010 at 11:00 AM EST
LC and LC-MS in the Pharmaceutical Industry: Small Molecule Quantitative Analysis and Matrix Effects
The constant production of new chemical entities by the pharmaceutical industry requires that new methods for quantitative determination of small molecules be continually developed to support the drug discovery and development pipeline. This webinar will cover general considerations for method development and validation, especially in support of ADME profiling, where samples are collected in complex biological matrices. Dr. Kevin A. Schug, Assistant Professor at the University of Texas at Arlington will discuss fundamental and applied studies of LC and LC-MS for trace quantitative analysis of small molecules and metabolites. A strong emphasis will be placed on bioanalytical method validation

ON DEMAND WEBCAST
Developing Better Methods for Reversed Phase HPLC (Part 2)
The Essential Guide from LCGC’s CHROMacademy presents Part 2 of our series on Developing Better Methods for reversed Phase HPLC. In this session, Kevin Schug (Chromacademy HPLC Department Dean) and Tony Taylor (CHROMacademy Technical Director), present more on logical optimization strategies for investigating and optimizing selectivity and resolution in reversed phase HPLC, all supported by Interactive Multi-Media from The CHROMacademy. The session will consider key topics such as Top Tips on Column Selection, Scouting Gradients to quickly decide between Isocratic and Gradient HPLC, Advanced pH Optimization techniques and Method Robustness, Introduction to Gradient Optimization and much more. A must see for everyone developing or optimizing methods for Reversed Phase HPLC.

ON DEMAND WEBCAST
Molecular Imaging for Localization and Distribution of Proteins, Peptides, Drugs and Metabolites by Mass Spectrometry
This presentation will detail the use of both MALDI-TOF and FTMS Mass Spectrometers to measure the tissue distribution of a variety of proteins, peptides, lipids, or small molecule drugs and metabolites in various tissues. Examples of tissue distribution for a variety of proteins in various tissues and simultaneous determination of the localization of small molecule drugs and metabolites administered at therapeutic dosage levels in time course studies will also be presented. These MS systems were tested to assess their capabilities in terms of accuracy, data quality, sensitivity, flexibility to analyze different types of molecules, automated analysis capabilities, and general utility in both small molecule and protein analyses. Results from various Mass Spectrometry technologies will be presented and compared.

Supercritical Fluid Chromatography in Theory and Practice
In this podcast series, we’ll be discussing how users can benefit from supercritical fluid chromatography (SFC) in their laboratories. Amongst its many advantages, this technology can be utilized in both the analysis and purification of various chemical compounds, and is regarded as a green technology by many companies actively pursuing greater efficiency in their labs.

High-Throughput Confirmation and Quantitation of a THC Metabolite in Urine
HPLC fluorescence detectors are used in a wide range of fields due to their excellent sensitivity and selectivity. Here, the performance attributes of a new high-sensitivity, ultrafast detector are examined.

Waters TargetLynx Matrix Calculator: A Tool for Robust Analytical Methods Development
The TargetLynx Matrix Calculator from Waters provides a convenient way to evaluate the matrix factor during the development of bioanalytical methods, to identify methods susceptible to matrix effects that could impact method robustness.

Standard and High-Speed Sample Enrichment and Clean-Up
Sodium adducts of sugars produce much better signal in LC-MS analysis (at least 10 times higher signal for the same sample). When glass bottles are used there is enough sodium leaching from the glass that it is possible to find very strong signals for the sodium adduct. This application note illustrates the importance of addition of microM amount of sodium to the mobile phase when sugars are analyzed.