This month, Dr. Charles White (YMC Europe GmbH, UK Operations) and Tony Taylor (Technical Director, Crawford Scientific), present an introductory guide to the theory, instrumentation and applications of Preparative HPLC
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Key Learning Objectives
Understand when to use preparative HPLC and have realistic expectations regarding yield
Impact of designing analytical HPLC methods for ultimate use in preparative scale separations
Appreciate selectivity is the key to unlock higher yield per injection
Adopt logical strategies and use equations to scale from analytical to prep.
Appreciate the practical issues encountered with scaling up, including solubility and loadability
Understand the equipment required for preparative chromatography and match column dimension to required yield
Consider method robustness and throughput as important variables in preparative chromatography
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