Understanding Gradient HPLC
  The CHROMacademy Essential Guide to Electrospray Ionization (ESI) for LC-MS (Part 1)  
LIVE WEBCAST
Thursday 24th February 2011, 11:00am EST;
16:00 GMT
 
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CHROMacademy announces the next in our highly popular series of FREE LIVE TUTORIAL events hosted in conjunction with the CHROMmunity

Understanding Electrospray Ionisation (ESI) for LC-MS

  • Overview of ESI Hardware
  • When to use electrospray
  • Eluents and eluent flow rate
  • The nebulization process – key factors in optimizing instrument response
  • Desolvation processes - ensuring efficient droplet desolvation
  • Mechanisms for producing ions in the gas phase – optimizing sensitivity
  • Quantitative aspects of ESI – droplet and gas phase processes leading to ion suppression / enhancement

Ask questions, share knowledge, join your colleagues in the Big Chromatography Conversations
WHO SHOULD ATTEND:
  • Anyone using LC-MS and wants to better understand Electrospray ionization, optimizing LC-MS instrument response or wanting to better understand how to avoid or overcome quantitative problems in LC-MS.
KEY LEARNING OBJECTIVES
  • Appreciate the needs to optimize analyte ionization state in solution for optimum instrument response
  • Understand the effects of eluent flow rate on droplet production at the capillary tip in modern ESI sources
  • Explore the spray processes that occur under various applied potential and how to identify a stable and non-stable spray
  • Investigate the processes involved in droplet desolvation, the effects of eluent composition and the principles involved in efficient droplet desolvation
  • Explore the theories and principles underlying gas phase ion production and highlight the ion production process from an analytical perspective
  • Consideration of the quantitative aspects of the ionization process, with respect to analyte solution chemistry and the processes which lead to ion suppression or enhancement
  • Overview of parameters that can be used to optimize the efficiency and reproducibility of the electrospray process
PRESENTERS:
Tony Taylor   Tony Taylor
Technical Director
Crawford Scientific
Dr. Amos Heckendorf   Kevin Schug
Assistant Professor
University of Texas at Arlington
Moderator:
Dr. Amos Heckendorf   Laura Bush
Editorial Director
LCGC
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