January 2012 Troubleshooting HPLC Mobile Phase and Pump Problems
VIEW RELATED ON DEMAND WEBCAST This month, Tony Taylor (Technical Director, Crawford Scientific) and Scott Fletcher (Technical Manager, Crawford Scientific), present the first in our series on HPLC Troubleshooting. In this session, we discuss practical troubleshooting and maintenance information around HPLC mobile phases and solvent delivery systems (pumps). In subsequent issues will hope to cover all of the major instrument and chemistry problems encountered in modern HPLC.VIEW ENTIRE TUTORIAL
Key Learning Objectives
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Identify good practices for preparation of HPLC eluents
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Appreciate the requirements for successful filtration and degassing of eluents
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Explore the links between HPLC eluent chemistry and chromatogram retention time / selectivity issues
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Appreciate the various designs of HPLC Solvent Delivery System (pump)
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Identify the most susceptible components of the pump
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Use tests and troubleshooting strategies to correctly identify pump issues
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Develop a maintenance schedule for your HPLC Solvent, Degasser and Pump
Also Included in This Tutorial
Related Materials from LCGC & CHROMacademy
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Check Valves – Troubleshooting
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LC Pumps – An Overview
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Ghost Peak Investigation in a Reversed_Phase Gradient LC System
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Troubleshooting Basics, Part I: Where to Start?
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Troubleshooting Basics, Part 3: Retention Problems