APRIL 2012
Troubleshooting HPLC Autosampler, Column and Detector Hardware Issues
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This month, Scott Fletcher (Technical Manager, Crawford Scientific) and Kevin Schug (Associate Professor, Universitry of Texas at Arlington), present the second in our series on HPLC Troubleshooting. In this session, we discuss practical troubleshooting and maintenance information around the hardware used for HPLC autosamplers and the UV detectors. We also consider the hardware issues associated with HPLC columns and column guards and filters. All issues are illustrated with real life examples and routine preventative maintenance discussed.
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Key Learning Objectives
• Learn different autosampler designs and injection valve configurations / operations
• Explore common autosampler hardware problems and associated chromatographic symptoms
• Appreciate the effect different sample solvents and injection volume on chromatographic performance
• Examine column hardware design, materials of construction and best practice for column protection
• Appreciate the requirement for column temperature control and chromatographic implications of poor temperature control
• Examine UV detectors and review common detector hardware problems
• Propose a maintenance schedule and several useful diagnostic tests

Also Included in This Tutorial
CHROMacademy Related Articles
• Autosampler Carryover – LCGC Magazine, LC Troubleshooting
• Column Care - LCGC Magazine, LC Troubleshooting
• Top 10 HPLC Column Myths – LCGC magazine
• Seeing is Believing: Detectors for HPLC - LCGC Magazine, LC Troubleshooting
CHROMacademy HPLC E-Learning
• HPLC Pump Systems
• Understanding HPLC Autosamplers
• Understanding HPLC Detectors
CHROMacademy Webcasts / Essential Guides Tools
• HPLC Troubleshooting - Eluents and Solvent Delivery Systems
• Stationary Phase Chemistry for Reversed Phase HPLC (Part I)
• Understanding HPLC Column Characterization and Selection
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