CHROMAcademy brings you a definitive guide on everything you need to consider for translating and transferring HPLC methods. The session includes hardware and data acquisition requirements for simple method transfer or translation to different column / particle size and morphology. We present worked examples of calculations, approaches and pitfalls associated with method scaling and transfer and include up to date concepts such as frictional heating and pressure based selectivity to ensure you are fully up to date in this subject area. A must see for everyone translating or transferring HPLC methods! VIEW RELATED ON DEMAND WEBCAST
Key Learning Objectives
•
Understand method transfer variables
•
Understand hardware requirements for running Fast HPLC methods
•
Investigate ‘geometrical scaling’ to convert flow rates and other method parameters between columns and particles of different sizes
•
Learn to convert gradient HPLC methods to maintain equivalent selectivity
•
Consider important factors in reverse scaling from UHPLC to HPLC methods
•
Investigate important aspects of temperature and pressure when scaling methods including Fractional Heating and Pressure Induced Selectivity changes
•
Build a portfolio of useful equations and rules of thumb for method scaling and transfer
Also Included in This Tutorial
Related Articles from LCGC
•
Method Translation in Liquid Chromatography
•
HPLC Method Translation and Rules of Thumb
•
Implementation of Methods Translation between Liquid Chromatography Instrumentation
•
Fast and Ultrafast HPLC on sub-2 μm Porous Particles — Where Do We Go From Here?
•
Challenging Beliefs About How Core-Shell Particles Work
Like us on Facebook
Follow us on Twitter
