MAY 2012
Troubleshooting HPLC Separations Part 1
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This month, Scott Fletcher (Technical Manager, Crawford Scientific) and Dr. Merlin K L Bicking (President, ACCTA Inc), present the third in our series on HPLC Troubleshooting. In this session, we discuss practical troubleshooting for HPLC separations, with special emphasis on problems with retention time variability, loss of efficiency and issues with peak shape. Problems are examined in terms of hardware and separation chemistry along with a host of practical tips and tricks to quickly identify and fix problems, and useful suggestions on how to avoid the problems in the first place!
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Key Learning Objectives
• Learn how to quickly and accurately identify the common causes of retention time, efficiency and peak shape issues
• How to identify / quantify chromatographic issues
• Appreciate how separation issues relate to mobile phase composition, temperature, column modification and age, sample solvent strength, sample preparation etc.
• Chromatographic and / or system maintenance in order to fix and prevent problems
• Discuss a preventative / good practice approach to avoiding separation issues from both methodological and hardware perspectives

Also Included in This Tutorial
CHROMacademy Related Articles
• Peak Shape Problems – What causes fronting and tailing
• HPLC variability and how to control it
• Broad Peaks – HPLC Troubleshooting
• HPLC Retention Time Changes
• Troubleshooting Basics, Part I: Where to Start?
CHROMacademy HPLC E-Learning Modules
• HPLC Pump Systems
• Understanding HPLC Autosamplers
• Understanding HPLC Detectors
CHROMacademy Webcasts / Essential Guides Tools
• HPLC Troubleshooting - Eluents and Solvent Delivery Systems
• HPLC Troubleshooting – Autosamplers, Columns and Detectors
• Mobile Phase Optimization Strategies for Reversed Phase HPLC
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Joe Furrevig, Editor contact


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