Developing Better GC methods - a blueprint

This month we explore what constitutes the optimum GC method. We examine instrument parameter settings as well as the chemistry and temperature program considerations which can and should be optimized for every analysis. This Essential Guide Tutorial analyzes what the ideal method specification might look like and highlights the important decisions which lead to highly stable and sensitive GC methods. Read the Tutorial...

GC column selection quiz

Find out just how much you know about GC columns and GC column selection.  Take the Quiz...

HPLC Pub Quiz

What you have to ask yourself is, do I feel lucky?
Well do ya' punk? -  Take the Quiz...

How much do you know about Gradient HPLC?

The gradient elution mode in HPLC can be very useful but how much do you really know about the mechanisms and manipulating gradients to effect a separation?  Find out with our quick 10 question quiz! Take the Quiz...

10 great chromatography troubleshooting tips

Establish a benchmarking method...
Don’t allow problems to start...

8 More...

HPLC Mobile Phases – 10 bad habits to avoid

1. Measuring the pH of the mobile phase after the organic has been added
2. Not using a buffer
3. Not using the buffer in its correct pH range
   
4. Adding buffer to organic  More...

What can you learn in 5min - SPE?

An SPE method follows 5 steps :

1. Sample pretreatment – e.g. pH adjustment, protein precipitation, filtration
2. Column conditioning – the column is made ready for the sample being loaded.  More...

10 Common GC Mistakes

1. Not Taking Enough Care at the Inlet
2. Turning the oven on before the carrier gas
3. Not programming in column dimensions
   More...

Troubleshooting retention time variability in HPLC

Everyone doing HPLC analysis has experienced drifting or unstable analyte retention times at some point – usually just as you’re about to head home!  Some quick tips on how to avoid the problems and improve method robustness  More...

10 points to understand Electrospray Ionisation

It’s often difficult in the busy laboratory to find time for leaerning and development.  CHROMacademy delivers key knowledge, quickly – and to prove it here is our 10 points to Understanding Electrospray Ionisation! More...