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Featured Application Note:
Automated Glycan Structural Isomer Differentiation Using SimGlycan Software
Julian Saba, Arun Apte, Ningombam Sanjib Meitei, and Rosa Viner
Here we present an automated workflow for complete structural elucidation of glycans by combining MSn fragmentation and data processing. The combination of permethylation, MSn, and SimGlycan software enabled successful identification and differentiation of various structural isomers of chicken ovalbumin released glycans. The simplified data analysis time was reduced to a matter of minutes, thus enabling truly automated, high-throughput data analysis. Read More |
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Using GC–MS Data-Mining Software to Reliably Estimate the Abundance of Identified Compounds
Gareth Roberts, ALMSCO International
This application note presents an innovative approach to GC peak integration, in which ions contributing to chromatographic peaks are intelligently distinguished from each other and from background interference. This results in cleaner spectra that allow accurate area determinations without recourse to time-consuming “quantion” analysis. Read More
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Using an In-house Hydrogen Generator to Replace Helium Carrier Gas for GC
Peter Froehlich, Parker Balston
Helium is commonly used as a GC carrier gas, however, its cost is increasing and its availability decreasing. In addition to the cost and availability issues, hydrogen can provide more rapid separations at lower temperatures than helium and provides longer column life. Hydrogen gas is obtained by the electrolysis of water and many laboratories supply it to the GC via an in-house H2 generator. Read More
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Sensitive Fluorescence Analysis of Aflatoxins Using Post-Column UV Derivatization on the Agilent 1260 Infinity LC
Udo Huber, Agilent Technologies
The analysis of aflatoxins in food matrices such as grain and nuts is an important task for the food industry to ensure product safety. This application note shows the analysis of the four major aflatoxins with post column UV derivatization and fluorescence detection. Read More
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Rapid Separation of Basic Drug Compounds Under Alkaline pH Conditions on pH-Stable, Hamilton PRP ™-C18
Derek Jensen and Mark Carrier, Hamilton Company
Mobile phase pH is a useful tool in methods development, particularly for separation of neutral forms of amines or other organic bases under alkaline conditions. In this study, separation of six basic drug compounds is achieved in under 6 min on a short (50 mm) PRP-C18 column using a generic, linear gradient. Read More |
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Age Determination of Parmigiano-Reggiano
Kendra L. Cox, PhD, Hitachi High Technologies America, Inc.
The production of parmigiano-reggiano is tightly regulated, resulting in a cheese of consistently high quality and cost. A lengthy aging process helps impart the cheese with a distinctive taste and texture, with additional value placed on cheeses aged over 18, 22, and 30 months. The maturation process includes enzymatic conversion of amino acids, and, as such, the levels of these amino acids have been found to be correlated with the age of the cheese. Presented here is a chromatographic method for amino acid analysis of parmigiano-reggiano using the Hitachi L-8900 Amino Acid Analyzer. Read More |
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Introducing the Hamilton PRP™-C18 - High-Efficiency Separations at Any pH
Derek Jensen and Mark Carrier, Hamilton Company
The Hamilton PRP-C18 is a new column designed to provide high-efficiency reversed phase separations over an extended column life in nearly any mobile phase or pH. The rigid stationary phase has excellent mechanical and thermal stability (>100 °C), does not experience shrinkage or swelling, and is completely inert to most conditions commonly encountered in reversed phase chromatography. In this study, the pH stability of the PRP-C18 is evaluated. Even after prolonged exposure to concentrated (1 molar) NaOH and H2SO4 there was no measurable deterioration in performance. Read More
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Chrome (VI) Analysis in Drinking Water
Maria Ofitserova, PhD, Pickering Laboratories, Inc., Pickering Laboratories, Inc.
Pickering Laboratories post-column derivatization system Pinnacle PCX was used to analyze chromium (VI) in drinking water according to EPA Method 218.7. Quantification levels of 0.01 µg/L were achieved with injection volumes as low as 100 µL. Read More
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Antibody Drug Conjugate Analysis
Wyatt Technology
A novel method for drug antibody ratio determinations based on SEC-MALS in conjunction with UV absorption and differential refractive index detection. Read More |
Molar Mass Determination of a Synthetic Rubber Resin via Single-, Dual- and Triple-Detector Gel Permeation Chromatography
Amandaa K. Brewer, Tosoh Bioscience
The most preeminent technique analysts use for the characterization of physicochemical properties of natural and synthetic rubbers is gel permeation chromatography (GPC). One of the advantages of GPC is that it can be used to provide molar mass averages and molar mass distributions of both the raw polymers used in the rubber product and the final compounded product. Here we demonstrate the expansion of the EcoSEC® GPC System, equipped with a dual flow RI detector for single-detector GPC experiments, to a dual- and triple-detector GPC system encompassing external VISC and MALS detectors for multi-detector GPC experiments. Read More |
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Drug Metabolite Detection and Identification
George McLeod, Sian Ives, and Thomas Amos Jacobsen, Bruker Daltonics
Characterization of the metabolites of drug candidates is a critical step in drug development. The observed activity and toxicity of a drug molecule may be severely impacted by metabolic modification. In this application brief, we demonstrate the use of software-based metabolite prediction coupled with high-performance Qq-TOF LC–MS and component-finding algorithms for the discovery and identification of metabolites of the well-known drug Verapamil. Read More |
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One-Step Extraction and Concentration for Identifying Pharmaceuticals and Personal Care Products in Water, Biosolids and Solids
Tom Hall, FMS, Inc.
The scope of human exposure to pharmaceuticals and personal care products (PPCPs) from the environment is complicated and increased monitoring is occurring to determine the effect on humans of long-term, low-level exposure to PPCPs. Due to their persistent nature and toxicity, monitoring water sources for PPCPs is a growing priority for both government agencies and consumers. The following procedure outlines the fully automated, sample-to-vial extraction and concentration of water matrices for the detection of these compounds in one rapid and efficient process. Read More |
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Extracting Semi-Volatile Organics from Drinking Water by EPA Method 525.3 Using the SmartPrep Cartridge Extractor
Brian LaBrecque, Horizon Technology Inc.
In 2012 the Federal Register released 525.3, which contains significant changes when compared to its predecessor. This application note will be used to showcase additional extraction conditions that can be used to decrease sample processing times. This would allow a laboratory to process more samples in less time.
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RediSep Rf Gold® C18Aq for Highly Aqueous Mobile Phases
Jack Silver, Teledyne Isco
RediSep Rf Gold® C18Aq media consists of C18 bonded to silica with modifications to show improved performance under conditions where the solvent contains a high concentration (>50%) of water. If compounds elute with more than 50% organic solvent, the RediSep Rf Gold C18 media shows improved performance. Purifications of water-soluble organic compounds are demonstrated. Read More |
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ChloroFiltr®: A Novel Sorbent for Chlorophyll Removal Using QuEChERS
Xiaoyan Wang, UCT, Inc.
Spinach and other highly pigmented vegetables contain chlorophylls, carotenoids, xanthophylls, and anthocyanins. Chlorophylls have the greatest adverse effect on GC system due to their non-volatile characteristics. This QuEChERS procedure uses ChloroFiltr® to significantly reduce chlorophylls without sacrificing the recoveries of planar pesticides. Read More |
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